探讨两种干燥方式下卡帕藻对5-氟尿嘧啶所致小鼠肠粘膜损伤的保护作用。将35只Balb/c小鼠随机分为5组：正常组（N）、模型组（M）、5-氨基水杨酸组（5-ASA）、晒干卡帕藻组（SKA）、晾干卡帕藻组（AKA）。采用腹腔注射5-FU构建肠粘膜损伤小鼠模型，每日记录小鼠体量和摄食量。实验结束后，统计胸腺和脾脏指数，观察空肠组织学，统计绒毛（villus）长度、隐窝（crypt）深度及两者比值（V/C值），使用酶联免疫法检测血清中TNF-α和IL-10的含量。研究显示，各受试物组的体重增长率高于M组（-3.30%、-6.19%、-5.09%>-7.50%），且第三天可显著抑制小鼠体重（16.85、16.83、16.76>15.75 g）和摄食量（2.15、2.18、2.19>1.54 g）的降低。5-ASA组（5.92 mg/10 g b.w.）和AKA组（6.81 mg/10 g b.w.）的胸腺指数略高于M组（5.67 mg/10 g b.w.）。组织学分析表明，与M组相比，各受试物组均可改善空肠组织学变化和显著上调绒毛长度（332.58、334.44、350.75>252.09 μm）（p<0.01）。另外，5-ASA组（6.39）和AKA组（6.35）的V/C值远高于M组（5.12）（p<0.05）。炎症因子方面，与M组相比，AKA组可显著降低血清TNF-α含量（470.73<580.69 pg/mL）（p<0.05）和提高IL-10含量（306.11>287.41 pg/mL）。因此，两种干燥方式下的卡帕藻均可改善5-FU诱导的小鼠肠粘膜损伤，其中晾干卡帕藻的效果更好。

Herein, the in vivo protective effects of Kappaphycus alvarezii, dried using two different methods, over 5-fluorouracil (5-FU)-induced intestinal mucosal injury were explored. A total of 35 Balb/c mice were randomly divided into five groups: non-treated group (N), placebo treated group (M), treated with 5-aminosalicylic acid (5-ASA) group, treated with sun-dried K. alvarezii (SKA) group, and treated with air-dried K. alvarezii (AKA) group. Intestinal mucosal injury was induced by intraperitoneal injection of 5-FU, and the body weight (bw) and food intake of the mice were recorded daily. After the experiment, the thymus and spleen indexes were calculated. The jejunum histology was evaluated, including the villus length, crypt depth, and their ratio (V/C value). An enzyme-linked immunoassay was performed to detect the serum levels of the tumor necrosis factor (TNF)-α and interleukin-10. Overall, the weight gain rates of all groups were higher than that of the M group (-3.30%, -6.19%, -5.09%> -7.50%). Moreover, the weight and food intake reduction was significantly suppressed by day 3 of treatment (16.85, 16.83, 16.76>15.75 g, and 2.15, 2.18, 2.19>1.54 g). The thymus index of the 5-ASA group (5.92 mg/10 g b.w.) and the AKA group (6.81 mg/10 g b.w.) were slightly higher than that of the M group (5.67 mg/10 g b.w.). Histological analysis showed that, compared with the M group, all treated groups had improved jejunum, with significantly longer villus (332.58, 334.44, 350.75>252.09 μm) (p<0.01). Additionally, the V/C values of the 5-ASA (6.39) and AKA (6.35) groups were much higher than that of the M group (5.12) (p<0.05). Compared with the M group, the AKA group has significantly lower serum TNF-αlevels (470.73<580.69 pg/mL) (p<0.05) and remarkably higher interleukin-10 (306.11>287.41 pg/mL). Altogether, dried K. alvarezii, regardless of the drying methods used, can alleviate the manifestations of 5-FU-induced intestinal mucosal injury in mice.

国家重点研发计划项目（2018YFC0311201）；山东省重点研发计划项目（2019GHY112058）