基于网络药理学探讨北沙参的抗炎机制

Mechanism of Anti-inflammatory Effect of Radix glehniae Based on Network Pharmacology

摘要:

北沙参为伞形科植物珊瑚菜的干燥根，药食两用，具有清肺热、止咳润肺的功能。现代研究表明，北沙参还具有抗菌、抗炎、抗氧化的功效。然而其抗炎功效的分子机制尚不明确。本研究运用TCMSP、PubChem、SEA、UniProt、GeneCards等数据库，收集北沙参所含成分中的活性化合物、活性化合物对应的靶点、抗炎作用相关靶点信息。进一步以北沙参的活性成分和抗炎靶点蛋白为网络节点构建"成分-靶点"网络，并进行基于String数据库的蛋白相互作用分析、GO生物功能注释和KEGG通路富集。结果经筛选和分析获得北沙参活性成分8个，包括槲皮素、β-谷甾醇、豆甾醇等。抗炎靶点92个，包括JUN、CASP3、EGFR、AKT1、HSP90AA1、TGFB1、PGR、MET、PLAU、PIM1、PIK3R1等，涉及雌激素信号通路，IL-17信号通路，EGFR酪氨酸激酶抑制剂耐药通路，AGE-RAGE信号通路，PI3K-Akt信号通路等与抗炎相关的信号通路。北沙参“多成分-多靶点-多通路”的作用特点，为进一步开展北沙参抗炎作用的机制研究提供了新路径和新方向。

Abstract:

Radix glehniae, the dry root of Glehnia littoralis, can be used as medicine and food. It has been used to clear lung heat, to relieve cough and to moisten lung in clinical application. Pharmacological research shows that it possesses anti-bacterial, anti-inflammatory and anti-oxidative effects. However, the molecular mechanism of its anti-inflammatory effect is still not clear. In this study, the databases of TCMSP, PubChem, SEA, UniProt, GeneCards were used to collect the active compounds of Radix glehniae, the corresponding targets of these active compounds and the anti-inflammatory targets. The molecular target network was also constructed using the active components and anti-inflammatory target based on the network nodes. Furthermore, the protein-protein interactions, GO enrichment and KEGG enrichment were analyzed based on the network node. Eight components, such as quercetin, β-sitosterol and stigmasterol were obtained as the active components of Radix glehniae. There were 92 anti-inflammatory targets, including JUN, CASP3, EGFR, AKT1, HSP90AA1, TGFB1, PGR, MET, PLAU, PIM1, and PIK3R1, which are involved in pathways, such as estrogen signaling pathway, IL-17 signaling pathway, EGFR tyrosine kinase inhibitor resistance pathway, AGE-RAGE signaling pathway, PI3K-Akt signaling pathway etc. The characteristics of multi-components, multi-targets and multi-pathways for Radix glehniae can provide a new way and direction for further study on anti-inflammatory mechanism.