短期口服菊粉提高小鼠的抗疲劳能力

Short-term Oral Administration of Inulin can Improve the Fatigue Resistance of Mice

摘要:

本文研究了短期食用菊粉对于小鼠抗疲劳能力的影响。通过不间断灌胃给予小鼠低（0.4 mg/kg）、高（2 mg/kg）剂量的菊粉45 d，在末次给药半小时后，测试了小鼠力竭游泳时间，强迫游泳后血清肌酸激酶（creatine kinase, CK）活性、血乳酸（lactic acid, LA）和血尿氮素（blood urea nitrogen, BUN）含量及小鼠粪便菌群丰度。结果显示，低剂量与高剂量组的力竭游泳时间与空白组相比分别提高45.62%与90.52%倍（p<0.05）。在剧烈运动后，与空白组相比，低剂量组与高剂量组小鼠血清中的CK活性分别下降为17.07% 与53.65% （p<0.05）；BUN含量分别下降68.70%与81.28%（p<0.05）；全血中的LA含量分别下降为16.29%与41.85（p<0.05）。在调节肠道菌群方面，相较于空白组，给药组肠道有害菌（变形菌门细菌）均有下调，其中高剂量组的菌群丰度最高。实验结果说明短期食用菊粉可以提高机体的抗疲劳能力，其作用机制可能与菊粉能调节肠道菌群丰度相关。

Abstract:

In this study, the effects of inulin on improving mouse's anti-fatigue and intestinal flora-regulating effects were investigated. The mice were administered orally at low (0.4 mg/kg) and high (2 mg/kg) doses of inulin for 45 days. Thirty minutes after the last administration, the time of weight-loaded swimming was recorded, and the serum creatine kinase (CK) activity, the blood urea nitrogen (BUN) and lactic acid (LA) contents and the fecal flora composition of mice were measured after the forced swimming. The results showed that the weight-loaded swimming times of the low-dose and high-dose groups increased by 45.62% and 90.52%, respectively, compared with the blank group (p<0.05). After strenuous exercise, compared with the blank group, the CK activities of the low-dose group and high-dose group decreased to 17.07% and 53.65% (p<0.05), respectively, with their BUN contents decreasing by 68.70% and 81.28% (p<0.05), respectively, and LA contents decreasing to 16.29% and 41.85% (p<0.05), respectively. In terms of regulating the gut microbiota, compared with the control group, the harmful bacteria (Proteobacteria) in the intestinaltract were down-regulated in the administration group. The experimental results showed that inulin has a significant anti-fatigue effect and regulatory effect on intestinal flora abundance.