绿茶富硒蛋白对肝癌HepG2细胞的抑制作用

Inhibitory Effect of Selenium-rich Protein in Green Tea on HepG2 Cells of Liver Cancer

摘要:

本研究探讨了绿茶富硒蛋白对肝癌HepG2细胞的抑制作用。采用DEEM培养基培养对数生长期的肝癌HepG2细胞，并将其作为对照组，将浓度分别为0.11 g/L、0.18 g/L、0.25 g/L、0.50 g/L的绿茶富硒蛋白为实验组；将添加二甲基亚砜（DMSO）的DMEM培养基作为参考组。通过光密度值计算抑制率，采用流式细胞仪检测凋亡率，并实验的检测细胞凋亡相关因子。结果表明：随着时间和绿茶富硒蛋白浓度的增加，肝癌HepG2细胞的抑制率越来越高，但对照组的抑制率始终为0。对照组肝癌HepG2细胞凋亡率约为6.17%，而当绿茶富硒蛋白浓度为0.50 g/L时，肝癌HepG2细胞凋亡率达到最大值30.60%。实验组凋亡因子表达水平整体呈下降趋势，Bcl-2和Bax的比值小于1，说明绿茶富硒蛋白浓度越高、培养时间越久，对肝癌HepG2细胞的抑制能力越强，且绿茶富硒蛋白可使S期细胞数目明显减少，由此说明绿茶富硒蛋白对肝癌HepG2细胞具有抑制作用。

Abstract:

The inhibitory effect of the selenium-rich proteins in green tea on HepG2 cells of live cancer was analyzed in this study. HepG2 cells in the logarithmic growth stage were incubated in the DEEM medium and used as a control group. The selenium-rich proteins of green tea at concentrations of 0.11 g/L, 0.18 g/L, 0.25 g/L and 0.5 g/L, respectively, were used as the experimental groups. The DMEM medium supplemented with dimethyl sulfoxide (DMSO) was used as the reference group. The inhibition rate was calculated by the optical density value, the apoptotic rate was detected by flow cytometry, and apoptosis-related factors were detected experimentally. The results showed that the inhibitory rate for the HepG2 cells of liver cancer increased with time and the concentration of selenium-rich proteins in green tea, but the inhibition rate for HepG2 cells for the control group was always zero. The apoptotic rate for the HepG2 cells of the control group was 6.17%, and the maximum apoptotic rate reached the maximum (30.60%) when the concentration of selenium-rich proteins of the green tea was 0.5 g/L. The expression level of apoptosis factors of the experimental groups showed an overall downward trend, the Bcl-2 and Bax ratio was lower than 1, indicating that the higher the concentration of the selenium-rich proteins of green tea, the longer the incubation time, the stronger the ability to inhibit HepG2 tumor cells, selenium-rich proteins. Therefore, green tea’s selenium-rich proteins can significantly decrease the number of S-phase cells, which indicates that the green tea selenium-rich proteins can inhibit HepG2 cells of liver cancer.