为了研究花椒麻素对2型糖尿病（type 2 diabetes mellitus，T2DM）小鼠糖脂代谢紊乱的治疗效果，采用高脂高糖饲料和链脲佐菌素（Streptozotocin，STZ）联合建立T2DM小鼠模型，设置空白组、模型组、花椒麻素高剂量（0.8 mg/kg·bw）、花椒麻素低剂量（0.4 mg/kg·bw）及阳性对照组，每组10只。实验期间测定小鼠的采食量、饮水量、体重和空腹血糖值，给药28 d后，测定小鼠的糖耐量，以及小鼠血清及肝脏的总胆固醇（TotalCholesterol，TC）、甘油三酯（Triglyceride，TG）、高密度脂蛋白（High-density Lipoprotein，HDL-C）、低密度脂蛋白（Low-density Lipoprotein，LDL-C）、丙二醛（Malondialdehyde，MDA）的含量并通过HE染色观察各组小鼠肝组织形态学变化。结果显示，花椒麻素可显著（p<0.05）降低血清中TG、TC、LDL-C及MDA含量，且显著（p<0.05）提高了HDL-C含量；高剂量组的肝脏TG、TC、及MDA含量分别比模型组显著（p<0.05）降低了28.31%、43.56%、52.14%，与阳性对照组作用相当；且形态学检测结果显示，经过28 d的给药治疗，与模型组相比，其高、低剂量组的肝组织损伤程度明显减轻，结果表明花椒麻素具有改善T2DM小鼠的糖脂代谢和氧化应激的作用。

To study the therapeutic effect of Zanthoxylum on glucose and lipid metabolism disorder in type 2 diabetes mellitus (T2DM) mice, the mouse model of T2DM was established by using high fat and high sugar feed and streptozotocin (STZ). The mice were divided into blank group, model group, high dose of Zanthoxydim (0.8 mg/kg bw), low dose of Zanthoxydim (0.4 mg/kg bw) and positive control (100 mg/kg bw) groups, with 10 mice in each group. The feed intake, water intake, body weight and fasting blood glucose of mice were measured during the experiment. Glucose tolerance (total cholesterol, TC), triglyceride (TG), high-density lipoprotein (HDL-C), low-density lipoprotein (LDL-C) and malondialdehyde (MDA) in serum and liver of mice were measured, and the morphological changes of mouse liver tissues in each group was observed by HE staining after 28 days of intragastric administration. The results showed that Zanthoxylum can significantly (p<0.05) reduce the contents of TG, TC, LDL-C and MDA in serum, and significantly (p<0.05) increase the content of HDL-C; compared with the model group, the contents of TG, TC and MDA in the liver of the high-dose group decreased significantly (p<0.05) by 28.31%, 43.56% and 52.14%, respectively, which were equivalent to those of the positive control group. And the morphological test results showed that after 28 days of treatment, compared with the model group, the degree of liver tissue damage in the high and low dose groups was significantly reduced. The results indicated that Zanthoxylum can improve glucose and lipid metabolism and stress response in T2DM mice.

贵州省科技计划项目（黔科合基础[2020]1Y431）；贵州省科技计划项目（黔科合平台人才[2018]5781-50）；贵州大学引进人才科研项目[贵大人基合字（2018）42号）]