Abstract:Hami melon is extremely prone to spoilage during storage and transportation. In this study, 3 strains of pathogens were isolated and purified using the tissue separation method from the Hami melon that decayed naturally during storage. Based on the identification by morphological observation, ITS sequencing analysis and phenotypic analysis, the CYS strain was Aspergillus ochraceus (JN942915.1), CJS was Trichothecium roseum (KM877467.1), and CWS was Diaporthe sp. (LT965977.1). The analysis of growth and metabolism of 3 strains of rot-causing pathogens with the Biolog-FF technique revealed that there were 44 main carbon sources of CYS metabolism, with the strongest ability to metabolize carboxylic acid followed by amino acids and sugars. There were 22 main carbon sources of CJS metabolism, with the strongest ability to metabolize amino acids followed by carboxylic acid and sugars. There were 41 main carbon sources of CWS metabolism, with the strongest ability to metabolize sugars followed by carboxylic acid and amino acids. Therefore, the metabolism of the pathogens existing on the surface and in the environment of fruits and vegetables could be accelerated due to the presence of substances conducive to the growth of these pathogens (such as sugars, amino acids and carboxylic acids), causing acceleration of the deterioration of fruits and vegetables. The conclusion of this study can further reveal the infection mechanism of pathogenic bacteria and provide a theoretical basis for the study of storage technology.
