[关键词]
[摘要]
本文研究了白藜芦醇改善γ射线诱导的T-淋巴细胞损伤大鼠的免疫功能。选取SPF级50只SD健康大鼠,随机分为空白组、模型组、低剂量组(20 μmol/L)、中剂量组(40 μmol/L)和高剂量组(80 μmol/L),建立γ射线诱导的T淋巴细胞损伤模型大鼠。建模成功后,对大鼠进行白藜芦醇灌胃处理,按实验设计对大鼠细胞T细胞增殖率、T淋巴细胞核因子-kB(Nuclear factor kB,NF-kB)表达、T淋巴细胞(CD3、CD4、CD8)、干扰素-γ(Interferon-γ,IF-γ)、白介素-12(Interleukin-12,IL-12)、白介素-4(Interleukin-4,IL-4)、白介素-3(Interleukin-3,IL-3)水平进行检测。结果表明,中剂量组(40 μmol/L)白藜芦醇的T细胞增殖率、CD3、CD4、CD8、CD4/CD8分别为2.15%、54.15%、35.11%、21.45%、2.11。白藜芦醇对T细胞分泌的IF-γ、IL-12、IL-4、IL-3的水平分别为21546.15 pg/mL、212.15 pg/mL、645.56 pg/mL、221.15 pg/mL,与其他实验组均呈显著差异(p<0.05),说明白藜芦醇能够明显抑制调节T细胞,改善大鼠的免疫功能。
[Key word]
[Abstract]
To study the effect of resveratrol on immune function of rats exposed to t-lymphocyte injury induced by tpa, 50 SPF-level SD healthy rats were randomly divided into blank group, model group, low-dose group (20 μmol/L), medium-dose group (40 μmol/L) and high-dose group (80 μmol/L), to establish the tlymphocyte injury model rats induced by γ-ray. After successful modeling, rats were given resveratrol by gavage. According to the experimental design, the proliferation rate of T cells, nuclear factor kB of T lymphocytes (NF-kB) expression, level of T lymphocytes (CD3, CD4, CD8), interferon-γ (IF-γ), interleukin-12 (IL-12), interleukin-4 (IL-4), and interleukin-3 (IL-3) were detected. The results showed that the proliferation rate, CD3, CD4, CD8 and CD4/CD8 of resveratrol group (40 μmol/L) were 2.15%, 54.15%, 35.11%, 21.45% and 2.11, respectively. The levels of IF-γ, IL-12, IL-4 and IL-3 secreted by T cells were 21546.15 pg/mL, 212.15 pg/mL, 645.56 pg/mL and 221.15 pg/mL, respectively, which were significantly different from those of other experimental groups (p<0.05). It is understood that resveratrol can significantly inhibit and regulate T cells and improve the immune function of rats.
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[基金项目]
江苏高校品牌专业建设工程资金项目(PPZY2015A097)