白藜芦醇改善γ射线诱导的T-淋巴细胞损伤大鼠的免疫功能

Resveratrol Improved the Immune Function of Rats Exposed to T-lymphocyte Injury Induced by Samray

摘要:

本文研究了白藜芦醇改善γ射线诱导的T-淋巴细胞损伤大鼠的免疫功能。选取SPF级50只SD健康大鼠，随机分为空白组、模型组、低剂量组（20 μmol/L）、中剂量组（40 μmol/L）和高剂量组（80 μmol/L），建立γ射线诱导的T淋巴细胞损伤模型大鼠。建模成功后，对大鼠进行白藜芦醇灌胃处理，按实验设计对大鼠细胞T细胞增殖率、T淋巴细胞核因子-kB（Nuclear factor kB，NF-kB）表达、T淋巴细胞（CD3、CD4、CD8）、干扰素-γ（Interferon-γ，IF-γ）、白介素-12（Interleukin-12，IL-12）、白介素-4（Interleukin-4，IL-4）、白介素-3（Interleukin-3，IL-3）水平进行检测。结果表明，中剂量组（40 μmol/L）白藜芦醇的T细胞增殖率、CD3、CD4、CD8、CD4/CD8分别为2.15%、54.15%、35.11%、21.45%、2.11。白藜芦醇对T细胞分泌的IF-γ、IL-12、IL-4、IL-3的水平分别为21546.15 pg/mL、212.15 pg/mL、645.56 pg/mL、221.15 pg/mL，与其他实验组均呈显著差异（p<0.05），说明白藜芦醇能够明显抑制调节T细胞，改善大鼠的免疫功能。

Abstract:

To study the effect of resveratrol on immune function of rats exposed to t-lymphocyte injury induced by tpa, 50 SPF-level SD healthy rats were randomly divided into blank group, model group, low-dose group (20 μmol/L), medium-dose group (40 μmol/L) and high-dose group (80 μmol/L), to establish the tlymphocyte injury model rats induced by γ-ray. After successful modeling, rats were given resveratrol by gavage. According to the experimental design, the proliferation rate of T cells, nuclear factor kB of T lymphocytes (NF-kB) expression, level of T lymphocytes (CD3, CD4, CD8), interferon-γ (IF-γ), interleukin-12 (IL-12), interleukin-4 (IL-4), and interleukin-3 (IL-3) were detected. The results showed that the proliferation rate, CD3, CD4, CD8 and CD4/CD8 of resveratrol group (40 μmol/L) were 2.15%, 54.15%, 35.11%, 21.45% and 2.11, respectively. The levels of IF-γ, IL-12, IL-4 and IL-3 secreted by T cells were 21546.15 pg/mL, 212.15 pg/mL, 645.56 pg/mL and 221.15 pg/mL, respectively, which were significantly different from those of other experimental groups (p<0.05). It is understood that resveratrol can significantly inhibit and regulate T cells and improve the immune function of rats.