变波长-梯度洗脱HPLC法同时测定杜仲的12种成分

Simultaneous Determination of 12 Active Components in Eucommia ulmoides Oliv. by Variable Wavelength-gradient Elution HPLC

摘要:

建立了变波长-梯度洗脱高效液相色谱法（HPLC）同时测定杜仲中的12种成分含量。色谱条件为：HiQ Sil C18色谱柱（250 mm×4.6 mm，5 μm），流动相为0.2%甲酸(A)-乙腈(B)梯度洗脱，检测波长为208 nm、277 nm和240 nm，方法学考察结果良好。用此方法测定不同生长年限、不同部位的杜仲样品中的桃叶珊瑚苷、京尼平、京尼平苷、京尼平苷酸、原儿茶酸、松柏苷、紫丁香苷、绿原酸、咖啡酸、松脂醇二葡萄糖苷、丁香脂醇二葡萄糖苷和橄榄树脂素含量。此方法所用3个波长既使各成分信号最大化又可以使基线稳定，梯度洗脱可以保证杜仲中的12种成分在60 min内全部出峰并完全分离，为HPLC测定杜仲中多种成分提供参考，进一步为杜仲中的功能性成分的含量测定和杜仲的质量控制提供有力的方法和手段。

Abstract:

A variable wavelength-gradient elution HPLC method for simultaneous determination of 12 components in Eucommia ulmoides Oliv. was established. Chromatography conditions were as follows: HiQ Sil C 18 column (250 mm×4.6 mm, 5 μm) was used. Mobile phase was 0.2% formic acid (A)-acetonitrile (B) with gradient elution. Detect wavelength were 208 nm, 277 nm and 240 nm. The result of methodology investigation indicated that this methd was good. Twelve components in Eucommia ulmoides Oliv. from different growth years and parts were determined using this method. This method could be used to determine the 12 components in Eucommia ulmoides Oliv within 60 min. Three wavelengths used in this method could not only maximize the signal of each component, but also stabilize the baseline. Gradient elution could ensure that all 12 components of Eucommia ulmoides Oliv. can be separated completely within 60 minutes, which will provide a reference for the determination of various components in Eucommia ulmoides Oliv. by HPLC. Furthermore, it will provide powerful method and means for the content determination of functional components and quality control of Eucommia ulmoides Oliv.