以柴达木大肥菇(Agaricus bitorquis（Quél.）Sacc)菌丝体为原料，基于超声波辅助提取手段，采用单因素和Box-Behnken法优化酚酸提取工艺，以大孔树脂纯化粗酚酸，并采用5种经典的体外抗氧化体系对纯化前后2种酚酸进行活性评价。结果表明：超声波功率130 W，乙醇浓度90%，提取温度61 ℃，提取时间30 min，液料比40:1（mL/g）为最佳提取工艺；体外抗氧化试验结果显示，在高浓度条件下，纯化酚酸（PPA）组对DPPH、ABTS自由基清除率分别高于对照组（Vc）4.31%、3.69%，也分别高于粗酚酸组（CPA）27.45%、32.45%，且PPA组的IC50值分别低于Vc组18.57%、5.20%，也分别低于CPA组40.78%、42.52%，其强弱顺序为：PPA>Vc>CPA；PPA组对羟自由基和超氧阴离子自由基清除率分别高于粗酚酸组（CPA）8.56%、30.09%，且PPA组的IC50值也分别低于CPM组17.39%、53.57%，强弱顺序为：PPA>CPA；PPA组还原能力高出CPM组51.05%；PPA组相对于CPA组具有较强的抗氧化活性。综上，柴达木大肥菇纯化酚酸具有较强的抗氧化活性，是天然抗氧化剂的良好来源。

The process of ultrasonic-assisted extraction was optimized by single factor experiment and Box-Behnken methods to extract phenolic acid from the mycelium of Agaricus bitorquis (Quél.) Sacc. The crude phenolic acid was isolated by macroporous adsorbent resins, and the antioxidant activity of two kinds phenolic acid was evaluated with five classical antioxidant assessment system. Results showed that the optimal conditions were as follows: ultrasonic power of 130W, ethanol concentration of 90%, extraction temperature of 61 ℃, extracting time of 30 minutes, and liquid/solid ratio of 40: 1(mL/g).The antioxidant activity experiment in vitro showed that the free radical scavenging capacity of DPPH and ABTS in purified phenolic acid (PPA) group were higher than those of Vc group (4.31% and 3.69%) and CPA group (27.45% and 32.45%) (p<0.05), and the half inhibitory concentrations in PPA group were lower than those of Vc group (18.57% and 5.20%) and CPA group (40.78% and 42.52%). The order of antioxidant ability was: PPA>Vc>CPA. The scavenging capacity of hydroxyl radicals and superoxide anion radicals in PPA group were higher than those of the CPA group (8.56% and 30.09%), and the half inhibitory concentrations in PPA group were lower than those of CPA group (17.39% and 53.57%). The order of antioxidant ability was: PPA>CPA. The reducing power of PPA group was comparable to that of Vc group, which was higher than that of CPA group (51.05%). The antioxidant activity of phenolic acid was increased with the increment of the sample concentration. In conclusion, this study revealed that the purified phenolic acid from the mycelium of Agaricus bitorquis (Quél.) Sacc have potent antioxidant effects, it could be used as a good source of natural antioxidant.

青海省国际合作项目（2018-HZ-801）；国家自然科学基金地区项目（31960471）