探究罗汉果提取物对甲状腺功能减退模型大鼠的干预效果及作用机制研究。选取50只SD健康大鼠，10只作为正常组，其余40只建立甲状腺功能减退模型，建模成功后分为模型组、阳性对照组（1 mL/100 g 0.08%的甲状腺片混悬液）、低剂量组（100 mg/kg罗汉果提取物）、高剂量组（160 mg/kg罗汉果提取物）各10只。检测各组大鼠下丘脑-垂体-甲状腺轴、下丘脑-垂体-性腺轴因子水平以及Shh信号通路基因mRNA表达量。研究结果表明，高剂量组大鼠游离三碘甲状原氨酸（FT3）、游离甲状腺素（FT4）、三碘甲状原氨酸(T3)、甲状腺素(T4)、促甲状腺素释放激素（TRH）分别上升至4.25 pmol/L、16.58 pmol/L、1.20 nmol/L、56.35 nmol/L和4.13 μIU/mL，促甲状腺激素(TSH)下降至1.96 μIU/mL，与其他组均有显著差异（p<0.05）。高剂量组雌性大鼠卵泡生成激素（FSH）、黄体生成激素（LH）、雌二醇（E2）、孕酮（P）水平分别上升至12.63 IU/L、14.35 IU/L、31.24 ng/L和186.59 ng/L，均显著高于阳性对照组、低剂量组（p<0.05）。高剂量组雄性大鼠FSH、LH、睾酮（T）分别达到了10.25 IU/L、12.35 IU/L和369.23 ng/L）。高剂量组大鼠Shh、Patched-1、Gli-1 mRNA表达量显著高于阳性对照组、和低剂量组（p<0.05）。说明罗汉果提取物可通过作用于Shh信号通路，调控异常的甲状腺生物学效应，改善甲状腺功能，增加甲状腺激素的分泌，维持TRH、TSH、甲状腺激素动态平衡。

The intervention effect and mechanism of Momordica grosvenori extract on hypothyroidism model rats was investigated in this work. Fifty healthy SD rats were selected as normal group, 10 as normal group, and 40 as hypothyroidism model. After successful modeling, the rats were divided into model group, positive control group (1 ml/100 g 0.08% thyroid tablet suspension), low dose group (100 mg/kg Momordica grosvenori extract), high dose group (160 mg/kg Momordica grosvenori extract), 10 rats each. The levels of hypothalamus-pituitary-thyroid axis, hypothalamus-pituitary-gonadal axis factor and the expression of Shh signaling pathway gene were detected. The results showed that the levels of free triiodothyronine (FT3), free thyroxine (FT4), triiodothyronine (T3), thyroxine (T4), thyrotropin releasing hormone (TRH) in the high dose group increased to 4.25 pmol/L, 16.58 pmol/L, 1.20 nmol/L, 56.35 nmol/L and 4.13 μIU/mL, respectively, and Thyrotropin (TSH) decreased to 1.96 μIU/mL, which was significantly different with other groups (p<0.05). The levels of follicle-forming hormone (FSH), luteinizing hormone (LH), estradiol (E2) and progesterone (P) of female rats in the high-dose group increased to 12.63 IU/L, 14.35 IU/L, 31.24 ng/L and 186.59 ng/L respectively, which were higher than those in the positive control group and the low-dose group (p<0.05). FSH, LH and testosterone (T) increased to 10.25 IU/L, 12.35 IU/L and 369.23 ng/L in the high dose group of male rats, respectively, which were higher than those in the positive control group and the low dose group (p<0.05). The mRNA expression levels of Shh, Patched-1, and Gli-1 in the high-dose group was higher than those of the positive control group and the low-dose group (p<0.05). Momordica grosvenori extract can regulate abnormal thyroid biological effects by acting on Shh signaling pathway, improve thyroid function, increase thyroid hormone secretion, and maintain the dynamic balance of TRH, TSH and thyroid hormone.

河北省科技计划项目（16277752D）