天麻提取物提高大鼠的抗缺氧能力

Improvement of Anti-hypoxia Ability of Gastrodia elata Extract in Rats

摘要:

研究天麻提取物对大鼠抗缺氧能力的作用机制。通过建立大鼠常压耐缺氧实验模型，随机分为正常组、阳性对照组、低剂量天麻提取物组和高剂量天麻提取物组,统计大鼠的体重、生存时间、生存率。检测丙二醛（MDA）、超氧化物歧化酶(SOD)、总抗氧化能力（T-AOC）、过氧化氢酶（CAT）、乳酸脱氢酶（LDH）、乳酸（Lactate，LA）、谷胱甘肽（GSH）、谷胱甘肽还原酶（GR）水平、热休克蛋白70（HSp70）、内皮素-1（ET-1）、一氧化氮合酶（iNOS）、缺氧诱导因子-1α（HIF-1α）相关蛋白表达。研究结果表明：高剂量天麻提取物组大鼠生存时间提高了34.01%，LDH、LA、GSH、GR水平分别为8614.24 nmol/mL、2.01 nmol/mL、965.5 mg/mL、0.30 U/mL，HSp70蛋白表达为4.14，均显著高于正常组（p<0.05）；MDA、CAT水平分别为63.52 U/mL、23.24 U/mL，ET-1、iNOS、HIF-1α蛋白表达分别为1.96、2.10、0.28，均显著低于正常组（p<0.05）。说明天麻提取物能够有效的提高大鼠抗氧化能力，改善大鼠的氧化应激相关指标水平，从而提高大鼠机体的缺氧能力。

Abstract:

To study the mechanism of Gastrodia elata extract on anti-hypoxia ability in rats. The rats were randomly divided into normal group, positive control group, low-dose Gastrodia elata extract group and high-dose Gastrodia elata extract group. The body weight, survival time and survival rate of rats were investigated. Malondialdehyde (MDA), superoxide dismutase (SOD), total antioxidant capacity (T-AOC), catalase (CAT), lactate dehydrogenase (LDH), lactate (LA), glutathione (GSH), glutathione reductase (GR), heat shock protein 70 (HSp70), endothelin-1 (ET-1), nitric oxide synthase (iNOS), the expression of related proteins for hypoxia inducible factor-1alpha (HIF-1a) were detected. The results showed that, compared with the normal group, the survival time of rats in the high dose Gastrodia elata extract group increased by 34.01%, the levels of LDH (8614.24 nmol/mL), LA (2.01 nmol/mL), GSH (965.5 mg/mL), GR (0.30 U/mL) increased (p<0.05), HSp70 (4.14), MDA (63.52 U/mL), CAT (23.24 U/mL), ET-1 (1.96), iNOS (2.10), HIF-1a (0.28) decreased (p<0.05). These results suggested that Gastrodia elata extract can effectively improve the antioxidant capacity of rats, the level of oxidative stress in rats, and thus improve the hypoxic capacity of rats.