本文研究了β-酪啡肽-7对老年小鼠肾功能可能的影响。50只雄性昆明小鼠，按组分别灌胃0.90%的生理盐水和低、中、高剂量的β-酪啡肽-7。连续灌胃30 d后，收集所有小鼠的血清及组织样本进行检测。组织病理学研究显示β-酪啡肽-7对老年小鼠的肾脏组织老化有一定改善作用，但高剂量组小鼠的肾小体肿胀似乎没有减轻，并且上皮细胞有坏死脱落现象。与模型组相比，中剂量组小鼠的血清肌酐（CRE）水平、肾脏组织中总抗氧化能力（T-AOC），超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GPx）活性分别增加了22.16%、69.02%、71.26%（p<0.05）和24.41%，而高剂量组分别增加了13.12%、64.88%、67.33%（p<0.05）和17.90%。提示β-酪啡肽-7可以调节老年小鼠的肾功能，增加其抗氧化能力，但不是剂量依赖式作用。此外，干预低中剂量β-酪啡肽-7小鼠的MDA含量都比模型组低（分别降低了15.40%、3.17%），但高剂量组反而提升了16.08%，说明高剂量组小鼠肾脏的氧化有所加重。上述结果表明，β-酪啡肽-7有改善老年小鼠肾功能异常的可能，可能通过提高抗氧化能力来改善衰老引起的老化损伤，但摄入过多β-酪啡肽-7似乎会加重肾脏负担。

The possible effects of β-Casomorphin-7 on renal function in aged mice were investigated. Fifty male Kunming mice were treated with 0.90% saline and low, moderate and high doses of β-Casomorphin-7. After continuous intragastric administration for 30 days, serum and tissue samples of all mice were collected for testing. Histopathological studies showed that β-Casomorphin-7 had a certain improvement effect on kidney tissue aging in aged mice. However, it seemed that the renal edema swelling in the high dose group was not alleviated and the epithelial cells were necrotic shedding. Compared with the model group, serum creatinine (CRE) levels, total antioxidant capacity (T-AOC), superoxide dismutase (SOD) and glutathione peroxidase in the middle dose group of mice (GPx) activity were increased by 22.16%, 69.02%, 71.26% (p<0.05) and 24.41%, respectively, while the corresponding values in the high dose group were increased by 13.12%, 64.88%, 67.33% (p<0.05) and 17.90%, respectively. These suggested that β-Casomorphin-7 can regulate renal function and increase its antioxidant capacity in aged mice, but it is not dose-dependent. In addition, compared with the model group, the MDA levels of the mice treated with the low and moderate doses of β-Casomorphin-7 were decreased respectively by 15.40% and 3.17%, but increased by 16.08% in high-dose group. It indicated that the oxidation of the kidneys in the high-dose group of mice was aggravated. The results suggest that β-Casomorphin-7 may improve the renal dysfunction in aged mice. It may improve the aging damage by improving the antioxidant capacity. However, it seems to increase the kidney burden and cause other disease with the excessive intake of β-Casomorphin-7.

江苏省高校自然科学研究项目（16KJB330011；17KJB190001）；扬州大学科技创新培育基金项目（2016CXJ107）；江苏省研究生科研与实践创新项目（XKYCX18-133）