本研究以‘鲁赫’刺蔷薇叶为原料，采用超声波辅助提取法，用不同溶剂（蒸馏水、无水乙醇、20%乙醇，40%乙醇，60%乙醇，80%乙醇，正丁醇、丙酮和乙酸乙酯）对叶中总黄酮、总多酚和原花青素进行提取，测定提取液的抗氧化活性（DPPH自由基清除能力、羟基自由基清除能力及总还原力），分析不同极性溶液对活性成分的提取效率，并评价抗氧化能力与活性成分的相关性。结果表明：不同极性溶剂提取效果不同，其中60%乙醇提取物具有最高的粗提物得率(23.76±0.04)%；60%乙醇提取物的总黄酮和总多酚含量均为最高，分别为74.29±0.01 mg/g和24.02±0.2 mg/g；80%乙醇提取物原花青素含量最高（12.92±0.46）mg/g。活性成分含量与抗氧化性之间呈正相关性。总多酚和总黄酮类化合物是‘鲁赫’刺蔷薇叶提取物抗氧化活性的主要贡献者。

In this study, the extraction of bioactive compounds from Rosa acicularis ‘Luhe’ leaves using ultrasonic-assisted method with different solvents (distilled water, absolute ethyl alcohol, 20% ethyl alcohol, 40% ethyl alcohol, 60% ethyl alcohol, 80% ethyl alcohol, 1-butyl alcohol, acetone and ethyl acetate) was studied. Total flavonoids, total phenols, procyanidins, and antioxidant activity (DPPH radical scavenging capacity, hydroxyl radical scavenging capacity and total reducing power) were first evaluated in the extracts of Rosa acicularis ‘Luhe’ leaves. Correlation analysis of phytochemical contents with antioxidant activity was evaluated. Results indicated that different solvent extraction effects were different. The extract with ethanol concentration of 60% V/V possessed the highest yields (23.76±0.04)% and the highest total flavonoids and total polyphenol content (74.29±0.01) mg/g and (24.02±0.24) mg/g, respectively. The highest procyanidins content (12.92±0.46) mg/g was obtained in ethanol concentration of 80% V/V. The bioactive compounds contents were positively correlated to the antioxidant activity. Total phenols and total flavonoids are the major contributors to the antioxidant activities of Rosa acicularis ‘Luhe’ leave extracts.

哈尔滨市科技创新人才研究专项资金（2017RALXJ001）；“十三五”国家重点研发计划项目（2016YFC0500307-07）