体外试验法模拟芒果中主要抗氧化物的相互作用关系

Study on the Interaction Relationship of Main Antioxidants in Mango Using Vitro Mimetic Experiment

摘要:

本文研究了芒果中所含的4种主要抗氧化物-没食子酸、芦丁、维生素C（Vc）、还原型谷胱甘肽（GSH）的抗氧化活性。应用体外试验法模拟芒果中的4种抗氧化物及其复配组合，通过分析各抗氧化物及其复配组合对羟自由基（·OH）、超氧阴离子（O2-·）、1,1-二苯基-2-苦基肼（DPPH）的清除能力大小来判断抗氧化能力的强弱。结果表明，·OH的清除率最高的是没食子酸+芦丁+GSH+Vc复配组，达99%，清除率最低组为单一抗氧物Vc组，较最高组低27%；O2-·清除率最高的是GSH+Vc复配组，达87%，而没食子酸+GSH+芦丁的复配组对O2-·清除率最低只有4%；DPPH清除率最高组为芦丁+GSH复配组合，为35%，Vc+没食子酸复配组对DPPH的清除率最低，为2.50%。综上所述，抗氧化物的抗氧化性可单独发挥作用，也可能是组合式发挥叠加作用，对自由基清除效果最佳的是GSH+Vc。

Abstract:

We have analyzed the antioxidant ability of four main antioxidants (gallic acid, rutin, vitamin C (Vc), glutathione (GSH)) of mango using vitro mimetic experiment and determined the influence of different antioxidant combinations on the scavenging capacities of hydroxyl radical (·OH), superoxide anion (O2-·) and 1, 1-diphenyl-2-picrylhydrazyl (DPPH). It showed that the highest scavenging rate of ·OH was gallic acid + rutin + GSH + Vc, which could reach 99%, however, the single Vc group was the lowest, 27% lower than the highest group. The highest scavenging rate of O2-· was GSH + Vc, which reached 87%, the complex group of gallic acid + GSH + rutin was only 4%. The rutin+GSH complex group had the highest clearance rate of DPPH (35%), while gallic acid + Vc was merely 2.50% on DPPH clearance rate. It indicated that the antioxidant capacity of the antioxidants may act alone or in combination, and the best effect on free radicals was GSH + Vc.