鹅去氧胆酸的大孔树脂纯化工艺优化及其降血脂活性

Optimization of Macroporous Resin Purification of Chenodeoxycholic acid and Preliminary Study on Its Lipid Lowering Function

摘要:

本文研究了大孔树脂对鹅去氧胆酸的纯化工艺及鹅去氧胆酸的降血脂功能。通过单因素法优选大孔树脂分离纯化鸡胆汁中的鹅去氧胆酸。将优化得到的鹅去氧胆酸灌胃给高血脂大鼠，给药28 d后测定对高血脂大鼠的血清总胆固醇，甘油三酯和高密度脂蛋白胆固醇含量的变化。结果发现：D101大孔树脂对鹅去氧胆酸有较好的吸附和洗脱效果，其最佳纯化条件是上样浓度为10 mg/mL，上样体积流量2 BV/h，7 BV55%乙醇洗脱，5 BV75%乙醇洗脱。经D101大孔树脂洗脱得到的鹅去氧胆酸纯度为98%。且得到的鹅去氧胆酸灌胃给大鼠后，给药的中、高剂量组显著降低了高血脂大鼠血清总胆固醇(TC)，甘油三酯(TG)和高密度脂蛋白胆固醇(LDL-C)的含量（p<0.05）。结果显示：该纯化工艺操作简单，环保无污染，可以得到纯度较高的鹅去氧胆酸，且药理实验证明得到的鹅去氧胆酸具有一定降血脂功效。

Abstract:

The purification process of deoxycholic acid and the hypolipidemic function of deoxycholic acid were studied in this paper. Single factor method was used to optimize the separation and purification of deoxycholic acid from chicken bile by macroporous resin. The changes of serum total cholesterol(TC), triglyceride(TG) and high density lipoprotein cholesterol (LDL-C) in hyperlipidemic rats were measured after the administration of the optimized goose deoxycholic acid to the hyperlipidemia rats after 28d. The results showed that D101 macroporous resin had better adsorption and elution effect on goose deoxycholic acid. The best purification conditions were 10 mg/mL, 2 BV/h, 7 BV55% ethanol elution and 5 BV75% ethanol elution. The purity of deoxycholic acid was 98% after elution by D101 macroporous resin. The contents of serum TC, TG and LDL-C were significantly reduced(p<0.05) in the middle and high dose groups of the hyperlipidemic rats fed with goose deoxycholic acid. The results showed that the purification process is simple, environmental pollution is free, the pure goose deoxycholic acid can be obtained, and the pharmacological experiment proved that goose deoxycholic acid has a certain effect on lowering blood lipid.