EDTA-壳聚糖对小鼠模型中Cd(Ⅱ)脱除作用

Removal of Cd (Ⅱ) from mouse model by EDTA-chitosan

摘要:

镉污染通过食物链传递严重威胁人类健康，因而探索安全、有效脱除生物体内镉的方法具有重要意义。本文制备得到乙二胺四乙酸(EDTA)酰化改性的壳聚糖衍生物(ETC)，并采用热分析技术表征了其稳定性。通过连续和不连续两种染毒方法，腹腔注射1 mg/kg CdCl2，建立Cd2+污染的小鼠模型。然后以ETC与EDTA和壳聚糖(CTS)等进行染毒小鼠脱镉，取小鼠眼球血、心、肝及肾脏器等，计算脏器系数及检测眼球中血、肝脏、肾脏、及心的镉含量。结果表明，不同染毒方式对小鼠体重及急性毒性作用存在显著差异。EDTA、ETC、CTS对血镉脱除率分别达58.94%、51.68%、37.43%；但ETC对小鼠各脏器镉的脱除率远高于其他脱镉剂，其中对心、肾镉脱除率分别高达63.90%、45.00%。相关研究结果为壳聚糖衍生物脱除重金属提供了一种参考方法。

Abstract:

Cadmium pollution through the food chain is a serious threat to human health, and it is thus of great significance to explore safe and effective ways to remove cadmium from organisms. In this paper, chitosan derivatives (ETC) were prepared via acylation with EDTA. The stability of ETC was characterized using TG-DSC technique. A mouse model of Cd contamination was established by intraperitoneal injection of 1 mg/kg CdCl2, according to two continuous and discontinuous exposure processes. The removal of cadmium in mice was determined when exposed to ETC, EDTA, and chitosan (CTS). The eyeballs were excised from living body. Heart, liver and kidney were excised by sacrificed mice. To track the cadmium in mice, the cadmium contents in blood, eyeball, liver, kidney, and heart were determined. The results showed that there were significant differences in the body weight and acute toxicity effects of different exposure methods on mice. The cadmium removal rates on the blood of EDTA, ETC, and CTS were 58.94%, 51.68%, and 37.43%, respectively. The removal rate of cadmium in mouse organs by ETC was much higher than that by other cadmium removal agents. The removal rates of cadmium in hearts and kidneys by ETC were 63.90% and 45.00%, respectively. The results provide a reference for the removal of heavy metals using chitosan derivatives.