瑞丽柠檬是国家地理标志产品，柠檬果实富含维生素、矿物质和类黄酮等物质，是一种营养和药用价值较高的水果。本文采用HPLC分析了云南湿热气候条件下栽培的云柠1号柠檬果实三个不同发育期具有降血脂、降血压和抗癌防癌功效的柠檬苦素和9种类黄酮物质，研究表明，柠檬苦素在果实发育过程中含量逐渐下降，但至花后210 d时，果实中柠檬苦素含量仍高出人体苦味阈值6.3倍；类黄酮中地奥司明含量最高，占类黄酮总含量的83.26%~84.61%，其次是以橙皮苷和新橙皮苷为主的黄烷酮类，占类黄酮总含量的14.18%~15.45%，含量最低的为多甲氧基黄酮，含量仅占类黄酮总含量的0.008%~0.89%，未检测出橘黄酮。推测云南湿热环境下有利于地奥司明成分的累积，类黄酮的累积转化机理有待进一步研究。

Ruili lemon is China geographical indication product with high nutritive and medicinal value, which is rich in vitamins, minerals, flavonoids and other nutritional substances. High-performance liquid chromatography was applied to determine Limonin and 9 flavonoids in lemon fruit widely cultivated in hot and humid climates of Yunnan Province. Limonin and Flavonoids exhibited a wide range of biological effects, including hypolipidemic effect, lowering blood pressure and anti-cancer. The results showed that there were significant differences in limonin and flavonoids at different Development stages.The content of limonin decreased gradually during the development stoags, but the content of limonin in the fruit was still higher than the threshold of human bitterness by 6.3 times in 210 days after the flowers. Diosmin in flavonoids was the highest among all maturity stages, which accounted for 83.26% ~ 84.61% of total flavonoids, followed by hesperidin and neo-hesperidin as the main flavanone accounting for 14.18%~15.45% of the total flavonoids. PMFs were detected at lowest levels in lemon fruit, which was only 0.008% ~ 0.89% of total flavonoids, and no tangeretin were detected. It is deduced that Yunnan hot and humid environment is conducive to the accumulation of diosmin components, but the accumulation and transformation mechanism of flavonoids needs further study.

现代农业（柑橘）产业技术体系专项（CARS-26）；公益性行业（农业）科研专项（201403036）；云南省创新人才培养对象（2017HB130）；云南省技术创新团队（2017HC023）；邓秀新院士工作站；德宏州中青年学术学科带头人（2015-4-4）