本文以循环伏安法在玻碳电极表面制备了聚吡咯（PPy）膜，以水热还原法制备了三维石墨烯（GN），并以此构建了甘油酶电极。所构建的甘油酶电极以甘油激酶（GK）和甘油三磷酸氧化酶（GPO）为催化剂，以三维石墨烯（GN）为载体，以聚吡咯（PPy）为介体，以Nafion溶液作为粘结剂。该甘油酶电极可以在酶、介体及电极表面提供良好的电子转移。论文探究了吡咯的聚合条件，并采用扫描电子显微镜及电化学方法对其进行了表征；对该酶电极的修饰材料、工作条件等进行了优化，采用电化学方法对其性能进行了评价。结果表明，聚吡咯的聚合圈数为8时其导电性能最优，所述的基于聚吡咯/石墨烯的甘油酶电极在pH为7.0，浓度为0.2 mmol/L的磷酸缓冲溶液对甘油有着较高的电流响应，其催化电流达46.2 μA，电流密度达677.6 μA/cm2。

Polypyrrole film (PPy) was prepared by cyclic voltammetry on the surface of glassy carbon electrode and three-dimensional graphene (GN) was prepared by hydrothermal reduction method in this study. A novel enzyme-based electrode was explored for the oxidization of glycerol through using glycerol kinase (GK) and glycerol triphosphate oxidase (GPO) as catalysts, three-dimensional graphene (GN) as the carrier, polypyrrole (PPy) as the mediator, and the Nafion solution as the binder. The glycerol enzyme-based electrode could provide good electrical transfer among the enzyme, mediator and the electrode surface. In this paper, the polymerization condition of pyrrole was investigated by scanning electron microscope and electrochemical methods. The modified materials and working conditions of the enzyme electrode were optimized and their properties were evaluated by electrochemical method. The results showed that the performance of modified electrode was optimal when polymerization cycle was 8, and the enzyme-based electrode conducted a good catalytic performance with a strong response for glycerol oxidization in 0.2 mmol/L phosphate buffer solution (PBS) at pH 7.0,. The peak catalytic current reached 46.2 μA and the current density reached 677.6 μA/cm2.

国家自然科学基金项目（21576101、21476087、21676106）；十三五国家重点研发计划子课题（2016YFB0101200（2016YFB0101204））；广东省自然科学基金项目（2017A030313045）