本文通过体外细胞实验观察研究6-姜烯酚诱导结直肠癌细胞凋亡及与APC（肿瘤抑制基因）表达的关系，以探讨6-姜烯酚抑制结直肠肿瘤的可能机制。首先通过体外细胞实验应用不同浓度6-姜烯酚（0、5、10、15和20 μM）分别对SW480进行干预诱导24 h，再利用高倍荧光显微镜观察细胞数目、形态变化，CCK8法测定细胞抑制率，Annexin V-FITC/PI流式细胞术检测细胞凋亡率及细胞凋亡周期，最后用Western-blot检测分析APC蛋白表达变化。结果显示，与对照组相比，6-姜烯酚可致使SW480细胞增殖周期阻断在G2/M期，并呈浓度依赖性地促进SW480凋亡，进而抑制细胞增殖，而且增强了APC表达（p<0.05）。因此，可以得出6-姜烯酚可诱导SW480凋亡从而抑制细胞增殖，并且可能与激活APC相关，随着这一研究的不断深入，6-姜烯酚作为临床潜在的抗肿瘤辅助药物，其抑制肿瘤细胞增殖将得到更深层次分子机制的支持。

The relationship between the apoptosis of human colorectal cancer cells (induced by 6-shogaol) and the expression of APC was investigated to study the possible mechanism of 6-shogaol inhibiting colorectal cancer in this study. SW480 was induced by different concentrations of 6-shogoal (0, 5, 10, 15 and 20 μM) in vitro for 24 h. Then the number and morphology of cells were observed by high-frequency fluorescence microscopy, the cell inhibition rate was determined by CCK8 assay, and the apoptosis rate and apoptosis cycle were detected by Annexin V-FITC/PI flow cytometry. In addition, the expression of APC protein was analyzed by western-blot. The results showed that compared with the control group, 6-shogaol could block the proliferation cycle of SW480 in G2/M phase and promote the apoptosis of SW480 in a concentration dependent manner, thereby inhibiting the cell proliferation and enhancing the expression of APC (p<0.05). Consequently, 6- shogaol could induce SW480 apoptosis and inhibit cell proliferation, and might be associated with the activation of APC. With the deepening of this study, the inhibition of tumor cell proliferation induced by 6-shogaol (used as a potential antitumor drug) would get more support of deeper molecular mechanism.

国家自然科学基金项目（81460369）；2017年宁夏“研究生教育创新计划”学位点建设项目（YXW2017014）；宁夏医科大学优势学科群项目（2001060304）