辣木籽酶解产物的蛋白回收率及DPPH•清除能力的研究

Protein Recovery and DPPH Radical Scavenging Activity of Enzymolyzed Products of Moringa Oleifera Seed

摘要:

以辣木籽为原料，采用水酶法制备辣木籽酶解产物；选用酶解产物的蛋白回收率和DPPH·清除能力为指标，通过单因素和响应面试验优化水酶法酶解辣木籽的工艺条件。首先通过单因素试验筛选辣木籽酶解的最佳用酶和酶解时间，单因素试验结果发现：Alcalas2.4L和Ns37071两种酶酶解得到的辣木籽酶解产物具有最佳的蛋白回收率和DPPH·清除能力，其蛋白回收率分别为45.65%和47.65%，DPPH·清除能力分别为99.88和93.89 μmol Trolox equiv/100 g；在次基础上进一步采用响应面优化酶解工艺，得其最佳酶解条件为：总加酶量为2.60%，Ns37071占总加酶量的46.00%，酶解24 h，此时辣木籽酶解产物的蛋白回收率为63.69±2.94%，DPPH·清除能力为139.38±0.96 μmol Trolox equiv/100 g。上述研究表明：水酶法可以高效酶解辣木籽，酶解产物具有较高的蛋白回收率和较强的DPPH·清除能力。

Abstract:

The enzymolyzed products of Moringa Oleifera seed were prepared by aqueous enzymatic extraction. The protein recovery and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity of the enzymolyzed products were selected as indexes to optimize the enzymatic hydrolysis conditions . Single factor experiment showed that the enzymolyzed products obtained by Alcalas 2.4L and Ns 37071 had the highest protein recovery and DPPH radical scavenging activity. The protein recovery were 45.65% and 47.65%, respectively, and the DPPH free radical scavenging activity were 99.88 and 93.89 μmol Trolox equiv/100 g, respectively. Further, the optimal enzymolysis conditions confirmed by response surface methodology were as follows: the total amount of enzyme 2.60%, Ns37071 accounted for 46% of the total amount of enzyme, and enzyme time 24 hours. The protein recovery was 63.69±2.94%, and DPPH radical scavenging activity was 139.38±0.96 μmol Trolox equiv/100 g. The study in this paper showed that the aqueous enzymatic extraction could efficiently hydrolyze Moringa oleifera seed, and enzymolyzed products had better protein recovery and DPPH radical scavenging activity.