本文研究木犀草素的肝保护作用。以人肝癌细胞HepG2为模型，以H2O2为氧化损伤来源，分为5个实验组包括空白组、损伤组、低浓度木犀草素保护组（0.5 μM+300 μM H2O2）、中浓度木犀草素保护组（5 μM+300 μM H2O2）和高浓度木犀草素保护组（10 μM+300 μM H2O2）测定细胞活力、活性氧（ROS）增长率、LDH、SOD和CAT酶活，测定细胞周期及细胞凋亡。经过10 μM木犀草素保护，细胞活力提高到84%左右，ROS的增长率与空白组无显著性差异（p>0.05），LDH、SOD和CAT酶活力显著低于损伤组（p<0.05）；流式细胞仪观察到随着木犀草素浓度增加，细胞的凋亡率减少并呈量效关系，而加药组的细胞周期与损伤组并无显著性差异（p>0.05）。木犀草素具有很好的肝保护能力，在食品、保健品和化妆品行业中具有潜在的应用价值。

The hepatocyte-protective effect of luteolin was investigated in this study. Human cancer cells HepG2 were used as the liver model and H2O2 was taken as the source of oxidative damage. The experiment was divided into five groups as follows: blank group, damaged group, the protective group with low concentration of luteolin (0.5 μM+300 μM H2O2), the protective group with medium concentration of luteolin (5 μM+300 μM H2O2) and the protective group with high concentration of luteolin (10 μM+300 μM H2O2). Cell viability, reactive oxygen species (ROS) growth rate, cell cycle and apoptosis, as well as lactate dehydrogenase (LDH), superoxide dismutase (SOD) and catalase (CAT) activities were measured. With the protection of luteolin, the cell viability of 10 μM luteolin-protective group reached 84%, and the enzyme activities of LDH, SOD and CAT were significantly lower than those of the damaged group (p<0.05). The increase rate of ROS had no significant differences with the blank group (p>0.05). The results of flow cytometer showed that apoptotic rate decreased in dose-dependent with the increase concentration of luteolin, but there was no significant difference on the cell cycle between the damaged group and the luteolin-protective group (p>0.05). Our study demonstrated that luteolin had a good hepatocyte-protective effect and had the potential for application in food, nutraceuticals and cosmetics industry.

国家自然科学基金项目（31301506）；广东省水利厅科技创新项目（2016-24）；华南理工大学“探索性实验”教学项目