以秀丽隐杆线虫（Caenorhabditis elegans，C. elegans）作为模式生物研究紫薯提取物（purple sweet potato extract，PSPE）对寿命的影响及其可能的作用机制。将秀丽隐杆线虫分别培养在普通培养基及添加不同浓度（70、140、280 μg/mL）PSPE的NGM培养基，研究PSPE对线虫寿命、体内脂褐素含量、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活力以及抗氧化相关基因表达水平的影响。结果显示，喂饲280 μg/mL PSPE的线虫比空白组线虫平均寿命延长33%，脂褐素水平降低30%，体内SOD和CAT抗氧化酶活力分别提高了17.7%和47.5%。饲喂PSPE后线虫体内daf-16、ctl-1和sod-3基因mRNA水平均有不同程度的提高，其中280 μg/mL PSPE组线虫体内daf-16基因转录水平提高23.2%，ctl-1基因转录水平提高37.4%，sod-3基因转录水平提高84.6%，而age-1基因转录水平呈下降的趋势，280 μg/mL剂量组降低了26.4%。

Effects of Purple sweet potato extract (PSPE) on the lifespan of Caenorhabditis elegans (C. elegans) and its possible mechanism were studied. C. elegans were fed in normal culture medium and NGM culture medium containing different concentrations (70, 140, 280 μg/mL) of PSPE to investigate the effects of PSPE on the lifespan of C. elegans as well as the content of lipofuscin, superoxide dismutase (SOD) and catalase (CAT) and the expressions of antioxidant-related genes in vivo. The Results showed that the average lifespan of C. elegans fed with 280 μg/mL PSPE increased by 33%, the accumulation of lipofuscin decreased by 30%, the activity of SOD and CAT increased by 17.7% and 47.5%, respectively, compared with the blank group. The mRNA levels of daf-16, ctl-1 and sod-3 in nematodes were increased in different degrees, and the mRNA expressions of daf-16, ctl-1 and sod-3 in 280 μg/mL PSPE group were increased by 23.2%, 37.4% and 84.6%, respectively; While the mRNA expression of age-1 in 280 μg/mL PSPE group was decreased by 26.4%.

国家自然科学基金应急管理项目（31540086）