电感耦合等离子体质谱法分析灵芝孢子油中的微量元素含量

Analysis of Trace Elements in Ganoderma lucidum Spore Oil by ICP-MS

摘要:

食品中的微量元素分析对食品安全控制与营养评价有着重要的意义，为正确评价灵芝孢子油的食用、药用价值提供参考。取市售灵芝孢子油，运用微波消解仪消解灵芝孢子油样品，消解过程采用硝酸+双氧水酸体系预处理，减少了酸的使用，消解更完全。利用电感合等离子体质谱仪（ICP-MS）在碰撞模式下测定灵芝孢子油中24种微量元素含量。实验结果表明，灵芝孢子油中含量丰富的有益微量元素，尤其以Ca、P、Mg、Fe、K和Zn的含量居多，其次为Na、Sn、Sr、Cr、Cu、B和Mn；有害微量元素Pb、As和Hg含量符合保健食品污染物限量要求。对于所测元素，Hg的线性范围为0~1 μg/L，其他元素的线性范围为0~100 μg/L，仪器检出限在0.03~7.0 μg/L之间，方法定量限在0.1~23.33 μg/kg之间，加标回收率在84.16%~107.00%之间，相对标准偏差0.83%~4.25%（n=6）之间。研究发现该方法精密度、灵敏度高，结果可靠。

Abstract:

The analysis of trace elements in food is necessary for food safety control and nutrition evaluation. This can provide a reference for the proper evaluation of the edible and medicinal value of Ganoderma lucidum spore oil. Commercial G. lucidum spore oil was digested using a microwave digestion system. Pretreatment with a nitric acid and hydrogen peroxide acid system was carried out for the digestion process. This reduced the use of acid and allowed the digestion to be more complete. The concentrations of 24 trace elements in G. lucidum spore oil were determined by inductively coupled plasma mass spectrometry (ICP-MS) in collision mode. The results indicated that G. lucidum spore oil was rich in calcium, phosphorus, magnesium, iron, potassium, and zinc, followed by sodium, tin, strontium, chromium, copper, boron, and manganese. Furthermore, the concentrations of the toxic trace elements lead, arsenic, and mercury were lower than the limits required for health food pollutants. For all measured elements, the linear range of mercury was 0~1 μg/L, and the linear ranges of other elements were 0~100 μg/L. The limit of detection for the instrument ranged from 0.03~7.0 μg/L. The limit of quantitation for the method was in the range of 0.1–23.33 μg/kg. The recoveries ranged from 84.16% to 107.00%, and the relative standard deviations were between 0.83% and 4.25% (n=6). In conclusion, the method demonstrates high precision and sensitivity, and yields reliable results.