核桃仁种皮多酚对免疫抑制小鼠的调节作用

Effect of Polyphenols from Walnut Kernel Pellicle on Immunosuppressed Mice

摘要:

为了研究核桃仁种皮多酚对环磷酰胺（CTX）所致免疫低下模型小鼠的免疫调节功能，通过腹腔注射环磷酰胺的方法建立免疫抑制小鼠模型，将小鼠随机分为5组：空白组、模型组、核桃仁种皮低、中、高剂量组，研究不同灌胃剂量的核桃仁种皮多酚对免疫抑制小鼠的脏器指数、吞噬指数、血液中相关指标、免疫球蛋白IgG、IgA、IgM以及LDH和ACP酶活力的影响。研究结果表明核桃仁种皮多酚具有一定的提高免疫力的作用，核桃仁种皮多酚能够改善试验小鼠的脏器指数，提高碳廓清指数，使淋巴细胞的增殖率提高，在一定程度上维持免疫抑制小鼠的血液细胞分布平衡，增加免疫抑制小鼠的免疫球蛋白含量，增强LDH和ACP酶的活力，维持免疫抑制小鼠生理指标的稳定，从而提高试验小鼠机体的免疫活性。

Abstract:

An immunosuppressed mouse model, established through intraperitoneal injection of specific pathogen-free mice with cyclophosphamide (CTX), was used to study the immunomodulatory effect of walnut kernel pellicle polyphenols (WKPs). The mice were randomly divided into five groups: normal group, model group, and low-dose (50 mg/kg), medium-dose (100 mg/kg), and high-dose (200 mg/kg) WKP groups. The effect of different doses of WKPs on the thymus and spleen indexes, phagocytic index, blood-related indicators, immunoglobulin (Ig) G, IgA, and IgM levels, and lactic dehydrogenase (LDH) and acid phosphatase (ACP) activities were investigated. Consequently, in the immunosuppressed mice, the WKPs had a definite effect on improving and enhancing immune functions, by improving the organ index, increasing the carbon clearance index, increasing the rate of lymphocyte proliferation, maintaining the balance of blood cell distribution to some extent, increasing the immunoglobulin content, enhancing the activities of LDH and ACP, and maintaining the stability of physiological indexes.