以2,4-二氯苯氧乙酸（2,4-Dichlorophenoxyacetic acid，2,4-D）处理为阳性对照，探讨1.50%壳寡糖和0.50%壳聚糖浸泡处理在改善乙烯褪绿引起的柑橘果实果蒂病害及果实失水方面的作用，并探讨其对果实基本贮藏品质和抗性相关指标的影响。结果表明，外源乙烯、50.00 mg/kg 2,4-D、1.50%壳寡糖和0.50%壳聚糖复合处理对蜜橘果实内部品质均无显著影响。但1.50%壳寡糖和0.50%壳聚糖能延缓乙烯褪绿引起的果实失重，且效果优于50.00 mg/kg 2,4-D处理。1.50%壳寡糖可以诱导乙烯褪绿蜜橘果皮PPO、POD、PAL、GLU活性以及总酚、类黄酮含量的显著增加，对果皮CHI活性有一定的诱导作用；0.50%壳聚糖对乙烯褪绿蜜橘果皮PPO、POD、PAL、CHI和GLU活性均表现出显著的诱导作用，对果皮总酚和类黄酮含量有一定的诱导作用；50.00 mg/kg 2,4-D处理仅表现出对果皮POD和PAL的显著诱导作用，对果皮PPO、GLU活性和总酚、类黄酮含量仅表现出一定的诱导作用。壳寡糖和壳聚糖在替代商业上柑橘褪绿技术中2,4-D的使用方面具有潜在的应用价值。

The effects of 1.50% oligochitosan and 0.50% chitosan treatments on the control of stem-end rot and fruit water loss caused by ethephon degreening and indices related to basic fruit storage and disease resistance were assessed using 2,4-dichlorophenoxyacetic acid (2,4-D) treatment as a positive control. The results indicated that external ethylene, 50.00 mg/kg 2,4-D, 1.50% oligochitosan, and 0.50% chitosan treatments had no significant adverse effects on the storage quality of citrus fruits. However, 1.50% oligochitosan and 0.50% chitosan were superior to 2,4-D to reduce fruit weight loss caused by ethylene degreening. Treatment with 1.50% oligochitosan significantly increased the activity of polyphenol oxidase (PPO), peroxidase (POD), phenylalanine ammonia-lyase (PAL), and β-1,3-glucanase (GLU). Furthermore, it improved the total phenolic and flavonoid content in the peel of ethephon-degreened citrus fruit and increased the activity of chitinase (CHI) to a certain extent. Treatment with 0.50% chitosan also significantly increased the PPO, POD, PAL, CHI, and GLU activities, and although not significant, it appeared to improve the total phenolic and flavonoid contents. In contrast, 50.00 mg/kg 2,4-D treatment only caused significant inductions of POD and PAL activities in the peel, but had limited effects to increase the activity of PPO and GLU as well as total phenolic and flavonoid content. In conclusion, oligochitosan and chitosan are potentially valuable to replace 2,4-D for commercial post-harvest citrus degreening.

国家自然科学基金青年科学基金资助项目（31401540）；重庆市博士后科研项目特别资助项目（Xm2014106）；中央高校基本科研业务费专项资金资助项目（SWU113047）；“十二五”国家科技支撑计划资助项目（2015BAD16B07）