不同蛋白源酶解产物对过氧化氢诱导损伤PC12细胞的保护作用研究

Neuroprotective Effects of Different Protein Hydrolysates against Oxidative Stress-induced Cytotoxicity in PC12 Cells

摘要:

本文以H2O2诱导损伤的大鼠肾上腺嗜铬细胞瘤PC12细胞为细胞模型，分别研究了三种蛋白源酶解产物（脑活素、鳕鱼蛋白肽和核桃蛋白肽）对H2O2诱导PC12细胞氧化损伤的保护作用。结果表明，H2O2的加入会使得PC12细胞的细胞数目减少，部分胞体皱缩成圆形或椭圆形，周围光晕消失，且随着H2O2浓度的增加，细胞存活率逐渐降低。脑活素可获得最好的PC12细胞损伤保护作用，核桃蛋白肽次之，而鳕鱼蛋白肽作用不明显；此外，结果表明脑活素、鳕鱼蛋白肽和核桃蛋白肽对受损伤细胞的存活和增殖也能起一定的改善作用。因此，核桃蛋白肽是一种改善脑部功能性障碍的潜在原料。

Abstract:

Here, the effects of three protein hydrolysates (cerebrolysin, cod peptide, and walnut peptide) on rat pheochromocytoma (PC12) injured by H2O2 was evaluated. The results showed that addition of H2O2 decreased the number of viable PC12 cells and altered cell morphology, resulting in shrunken circular or oval shapes accompanied by disappearance of the peripheral halo. However, cerebrolysin exhibited the strongest protective effects against H2O2-induced oxidative injury in PC12 cells, followed by walnut peptide, whereas no significant effect was observed in the presence of cod peptide. Additionally, all three hydrolysates supported proliferation and survival of the injured cells. These findings indicated that walnut peptide constituted a potential raw food material capable of improving cerebral dysfunction.