采用乙腈/水溶液（84+16）提取试样、MycoSpin 400多功能柱净化，建立了发酵茶（普洱茶、湖南黑茶和红茶）中黄曲霉毒素B1（AFB1）、黄曲霉毒素B2（AFB2）、黄曲霉毒素G1（AFG1）、黄曲霉毒素G2（AFG2、赭曲霉毒素A(OTA)五种真菌毒素的简单、快速超高效液相色谱-串联质谱检测方法。试样以Agilent Zorbax Rrhd SB-C18柱分离，多反应监测(MRM)模式进行定量与定性分析，外标法定量。结果表明，在优化的条件下，五种真菌毒素在各自的线性响应范围内线性关系良好，相关系数(r)不低于0.9983，定量限（LOQ）为0.1~0.5 μg/kg，低、中、高3个加标水平的平均回收率（n=3）为63.5%~125.0%，相对标准偏差（RSD）为3.9%~17.2%。该方法前处理简单、检测速度快、净化效果好、结果准确、可靠，适合发酵茶中多种真菌毒素的快速检测。

A method for the determination of five mycotoxins in fermented Pu'er tea, and Hunan dark tea and black tea samples was established. Ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was used to identify aflatoxin B1 (AFB1), aflatoxin B2 (AFB2), aflatoxin G1 (AFG1), aflatoxin G2 (AFG2), and ochratoxin A (OTA) under the multiple reaction monitoring (MRM) mode. Tea samples were extracted with an acetonitrile/water solution (84 / 16), and purified using a MycoSpin 400 multifunctional clean-up column. Samples were separated on an Agilent Zorbax Rrhd SB-C18 column (2.1 mm ? 100 mm, 1.8 μm), and qualitative and quantitative analyses were performed using MRM mode. Quantitation was conducted using the external standard method. The results indicated that, under the optimal conditions, five mycotoxins showed a good linear relationship within their own linear response range, with correlation coefficients (r) more than 0.9983, and limits of quantification (LOQ) ranging from 0.1 to 0.5 μg / kg. The recovery of the five mycotoxins at low, medium, and high spiking levels ranged from 63.5% to 125.0%, and the relative standard deviation (RSD, n=3) values were between 3.9% and 17.2%. This method has a simple pretreatment, can provide rapid detection, good purification, and accurate and reliable results, and is suitable for the rapid detection of multi-component mycotoxin contaminants in fermented tea.

广东省高等学校优秀青年教师培养计划资助项目(Yq2013196)；国家质检总局科技计划项目(2014QK051)；广东省食药局科技计划项目（2015ZX07）；中山市科技计划项目（2014A2FC247、2015B2297）