葡萄籽原花青素的体内降血糖作用研究

Hypoglycemic Effect of Grape Seed Proanthocyanidins in Diabetic Mice

摘要:

本文研究了原花青素的体内降血糖作用。通过腹腔注射链脲佐菌素建立小鼠糖尿病模型，检测不同剂量原花青素（150 mg/kg、100 mg/kg、50 mg/kg）对糖尿病小鼠的体重、空腹血糖（GLU）、糖耐量、血清甘油三酯（TG）、总胆固醇（TC）、超氧化物歧化酶（SOD）、脂质过氧化物丙二醛（MDA）以及糖尿病小鼠盲肠中短链脂肪酸含量等指标的影响。结果表明：低中高剂量原花青素分别增加糖尿病小鼠体重13.23%、20.85%、30.03%；降低空腹血糖10.96%、17.18%、20.89%；显著改善糖耐量（p<0.01）；高剂量原花青素降低糖尿病小鼠血清TC 18.03%、TG 32.54%、MDA 22.88%，提高SOD活力30.28%；各剂量原花青素均可显著增加糖尿病小鼠盲肠内容物中总短链脂肪酸的含量（p<0.05或p<0.01）。因此原花青素具有体内降血糖，减轻自由基损伤、抑制脂质过氧化，进而改善糖尿病小鼠的糖、脂代谢异常的作用，这一研究发现将对人类血糖异常的控制提供有效途径，对于人体健康具有重要意义。

Abstract:

The hypoglycemic effect of proanthocyanidins was investigated. A diabetic model was established by intraperitoneal injection of streptozotocin (STZ) in mice, and the effect of proanthocyanidins at doses of 50 mg/kg, 100 mg/kg, and 150 mg/kg on the weight of diabetic mice, fasting blood glucose (GLU), glucose tolerance, serum triglyceride (TG), total cholesterol (TC), superoxide dismutase (SOD), lipid peroxide malondialdehyde (MDA), and the concentration of short chain fatty acids was determined. The results revealed that low, medium, and high dose of proanthocyanidins reduced body weight of diabetic mice by 13.23 %，20.85 %, and 30.03 %, respectively; lowered fasting glucose by 10.96 %, 17.18 %, and 20.89 %, respectively, and improved glucose tolerance (p<0.01). High doses of proanthocyanidins effectively decreased serum TC, TG, and MDA by 18.03 %, 32.54 %, and 22.88 %, respectively, while increased the serum SOD activity by 30.28 %. All the three doses of proanthocyanidins significantly increased the levels of total short chain fatty acids (p<0.05 or p<0.01).Thus, proanthocyanidins could reduce the serum sugar level, reduce the free radical damage, inhibit lipid peroxidation, and improve sugar and lipid metabolism in diabetic mice. Proanthocyanidins may play an important role in human health.