本研究以52?白酒灌胃小鼠，建立酒精肝损伤模型，以枳椇果梗为原料，经乳酸菌发酵制成枳椇乳酸菌发酵饮料后灌胃小鼠，然后通过测定小鼠血清和肝脏中特定酶的活性以及肝组织病理学显微切片评价枳椇乳酸菌发酵饮料对酒精肝损伤小鼠肝脏的保护作用。结果表明，灌酒引起小鼠血清谷丙转氨酶（ALT）和谷草转氨酶（AST）活性升高，灌胃枳椇饮料后小鼠血清ALT和AST活性显著降低。与模型组相比，枳椇乳酸菌发酵饮料试验组的小鼠肝组织超氧化物歧化酶（SOD）、乙醇脱氢酶（ADH）活性以及还原型谷胱甘肽（GSH）含量显著升高，氧化产物丙二醛（MDA）含量显著降低。肝组织病理检查结果显示，枳椇乳酸菌发酵饮料可明显抑制酒精肝损伤小鼠的肝肿大、脂肪变性。枳椇乳酸菌发酵饮料对酒精肝损伤小鼠肝脏有明显的保护作用，其功效与阳性对照药物联苯双酯相当，可作为一种解酒护肝型健康饮品。

A model of alcohol-induced liver damage was established in this study by administering 52° liquor to mice orally. A lactic acid-fermented beverage was prepared with the peduncles of Hovenia dulcis as the raw material, and was also administered to mice by gavage. The hepatoprotective effect of this lactic acid fermented beverage from the peduncles of Hovenia dulcis (LBPHD) was evaluated by measuring the specific enzyme activities in the serum and liver of the mice and liver histopathological analysis with microscopic sections. The results showed that the serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels were substantially elevated by the administration of alcohol, and were significantly decreased by the gavage administration of LBPHD. Compared with the model group, LBPHD could also increase the levels of superoxide dismutase (SOD), alcohol dehydrogenase (ADH), and glutathione (GSH), and decrease the level of malondialdehyde (MDA)-the oxidation product-in the liver tissues of mice. Liver histopathology results indicated that LBPHD could effectively alleviate hepatomegaly and steatosis in mice with alcohol-induced liver damage. These results demonstrate that LBPHD exhibits a significant hepatoprotective effect, which is comparable to that of biphenyldicarboxylate (the positive control). Therefore, LBPHD can be a healthy drink to counter the effect of alcohol and to protect liver tissue.

陕西省农业科技创新项目（2012NKC02-02）；杨凌国家级农业高新技术试验示范区科技专项（K33602140）