本文采用结肠癌细胞模型，探究了增强苹果来源二氢查尔酮对结肠肿瘤抑制功效的方法。一方面，采用MTT法考察了根皮素及其类似物对结肠癌细胞增殖的抑制作用，发现200 μmol/L根皮素对HT29、SW620与HCT116三种结肠癌细胞作用72 h的抑制率分别为74.18%、52.91%与72.78%，抑制活性与普遍性强于多种二氢查尔酮类似物，并呈现剂量与时间效应。另一方面，通过根皮素与多种果蔬功效成分的联合作用，发现在SW620与HCT116细胞中，根皮素与白藜芦醇及姜黄素之间存在相互作用。尤其对HCT116细胞，根皮素与姜黄素联合使用时的IC50值分别为69.78 μmol/L与4.27 μmol/L，显著低于单独使用时的153.88 μmol/L与8.80 μmol/L，两者以20:1的剂量比使用时，其联合指数均小于1，表现为协同效应，且在低浓度时更为显著。本研究为深入研究苹果二氢查尔酮的结肠癌抑制功效奠定了理论基础，并为指导消费者合理搭配膳食提供了科学依据。

CellsColon cancer cell lines were used to explore methods of enhancing the anti-tumor activity of apple dihydrochalcone against colon cancer. The growth inhibitory effects of phloretin and its analogues were measured using the MTT assay. The tumor inhibition rates of HT29, SW620, and HCT116 cells treated with 200 μM phloretin were 74.18%, 52.91%, and 72.78%, respectively, and phloretin showed stronger and braoder inhibitory activity than many of its dihydrochalcone analogues in a dose- and time-dependent manner. Furthermore, the interaction effects between phloretin and other different functional compounds in fruits and vegetables were investigated. Combinations of phloretin with resveratrol or curcumin showed synergistic inhibition against both SW620 and HCT116 cells. Particularly, in HCT116 cells, the half-maximal inhibitory concentration (IC50) values for phloretin and curcumin when they were used simultaneously were 69.78 μM and 4.27 μM, respectively, which were dramatically lower than when phloretin or curcumin was used alone (153.88 μM and 8.80 μM, respectively). When these two components were used at a dose ratio of 20:1, all combination indices were lower than 1, and a synergistic effect was observed; this effect was greater at low concentrations. Our results provide a theoretical basis for further studies of the inhibitory activities of apple dihydrochalcone against colon cancer cells, and they can be used to guide consumers in maintaining a balanced diet.

国家自然科学基金海外学者合作项目（31428017）；中国农业科学院基本科研业务增量项目（2014ZL041）