两种检测糖化血红蛋白(HbA1c)方法的比较

Comparison of Two Methods for Glycated Hemoglobin (HbA1c) Measurement

摘要:

血液中糖化血红蛋白（HbA1c）水平是衡量人体内长期血糖状态的标准。国际临床化学和实验医学联合会（IFCC）将高效液相离子交换层析（HPLC）作为检测糖化血红蛋白的标准参考方法；而20 mmol/L NaOH（阴极电解液）和20 mmol/L H2SO4（阳极电解液）形成的稳定pH梯度及含两性电解质的凝胶使毛细管等电聚焦电泳（cIEF）在窄PH梯度下对糖化血红蛋白有着显著的分离效率;本文将对HPLC与凝胶cIEF进行分析比较。采用HPLC和凝胶cIEF方法分别测定随机46例血样样品的HbA1c值，对两组测定结果进行相关性分析。表明两种测定方法相关性良好；但是，由于HPLC方法易受血红蛋白变异体干扰，特异性较低；cIEF不受血红蛋白变异体干扰，特异性高。因此，凝胶cIEF方法可以作为HPLC的替代方法应于糖化血红蛋白的标准检测

Abstract:

The level of glycated hemoglobin (HbA1c) in the blood is a standard measure of the long-term glycemic status of the human body. High-performance liquid chromatography (HPLC) is the standard reference method for HbA1c measurement as designated by the International Federation of Clinical Chemistry and Laboratory Medicine (IFCC). Gel-capillary isoelectric focusing (cIEF) with a stable pH gradient of 20 mmol/L NaOH (catholyte solution) and 20 mmol/L sulfuric acid (anolyte solution) and gels containing ampholytes have outstanding separation power. In this study, HPLC has been compared to gel-cIEF for the measurement of HbA1c. HPLC and gel-cIEF were used to measure the HbA1c values of 46 randomly selected blood samples. Correlation analysis revealed that the two methods showed a good correlation. The HPLC method was prone to interference by hemoglobin variants and exhibited low specificity. The gel-cIEF method was not affected by hemoglobin variants and showed high specificity. In conclusion, gel-cIEF can be used as an alternative method to HPLC for the standard detection of glycated hemoglobin.