马尾藻岩藻聚糖硫酸酯对高脂血症小鼠抗脂质过氧化的作用

Anti-lipoperoxidation Effects of Sargassum Fucoidans in Hyperlipidemic Mice

摘要:

本文研究了中、低分子量岩藻聚糖（MMWF1、MMWF2、LMWF1、LMWF2）四个不同组分马尾藻岩藻聚糖硫酸酯(SF)对高胆固醇血症小鼠脂质过氧化指标的影响。建立高脂血症小鼠模型。测定血清和肝脏中的脂质过氧化指标，包括丙二醛（MDA）含量、总超氧化物歧化酶(SOD)活性、过氧化氢酶（CAT）活性、过氧化物酶（POD）活性、谷胱甘肽过氧化物酶（GSH-PX）活性。实验表明：四组SF都能提高血清和肝脏中CAT、GSH-PX、SOD、POD活性;都能使血清和肝脏中MDA含量降低，其中高剂量组达到极显著水平（p<0.01），都能提高脂质代谢紊乱小鼠机体的抗氧化能力，恢复机体内氧化系统平衡，降低脂质过氧化的产生，保持机体内脂质代谢相关酶的活力，改善并恢复脂质正常代谢。四组SF抗脂质过氧化能力依次为：MMWF2>MMWF1>LMWF2>LMWF1，MMWF抗氧化能力比LMWF强，相似组分间硫酸根含量越高的抗氧化能力越强。

Abstract:

The effects of four fractions of Sargassum fucoidans (SF) with medium and low molecular weights (MMWF1, MMWF2, LMWF1, and LMWF2) on lipid peroxidation indexes in hyperlipidemic mice were investigated in this study. A mouse model of hyperlipidemia was established. The lipid peroxidation indexes, malondialdehyde (MDA) content, and activities of superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), and glutathione peroxidase (GSH-PX) in the serum and liver were measured. The results showed that all four SF fractions increased the activities of CAT, GSH-PX, SOD, and POD and decreased the MDA content in the serum and liver at a significant level (p < 0.01) with the high-dose treatments. All four fractions of SF improved anti-oxidation capacity, recovered the balance of the oxidation system, decreased lipid peroxidation, maintained the activity of enzymes related to lipid metabolism, and improved and recovered normal lipid metabolism in hyperlipidemic mice. The anti-lipoperoxidation capacities of the four SF fractions were as follows: MMWF2 > MMWF1 > LMWF2 > LMWF1. The anti-lipoperoxidation capacity of MMWF was greater than that of LMWF, which indicated that among similar fucoidans, increased sulfate content resulted in higher antioxidant capacity.