本文采取微波提取法从香蕉皮中提取得到黄酮粗提物，利用H103大孔树脂对提取物进行纯化，与丙酸复配得到复配剂。以香蕉果皮黄酮-丙酸复配剂为受试物，根据国家卫生部发布的《食品安全性毒理学评价程序和方法》对其进行毒理学试验，以便在生产实践中推广应该。利用小鼠为试验对象，进行了急性毒性试验（LD50）、骨髓细胞微核试验、小鼠精子畸形试验以及哺乳动物微粒体酶试验（Ames试验），对复配剂的毒理性进行了研究。结果表明，雌小鼠LD50为10.80 g/kg BW，可信限为7.41~15.70 g/kg BW；雄小鼠LD50为12.60 g/kg BW，可信限为7.75~20.50 g/kg BW；骨髓细胞微核试验、精子畸形试验、Ames试验的结果都为阴性，表明香蕉果皮黄酮-丙酸复配剂对小鼠体细胞和生殖细胞无诱变作用，也无直接或间接的致突变作用, 属实际无毒级物质。香蕉果皮黄酮-丙酸复配剂可作为食品防腐添加剂。

Crude flavonoid extract was obtained from banana peel by microwave extraction and purified using H103 macroporous resin and combined with propionic acid to produce flavonoid-propionic acid mixtures. Toxicology testing was conducted using these mixtures as per the Procedures for Toxicological Assessment of Food published by Ministry of Health of The People's Republic of China. Toxicology testing included using the acute toxicity test for minimum lethal dose for 50% mice (LD50), bone marrow cell micronucleus test, mice sperm morphology test, and Ames test. The results showed that the LD50 values for female and male mice were 10.8 and 12.6 g/kg body weight (BW), respectively; while the fiducial limits for female and male mice were 7.41~15.7 and 7.75~20.5 g/kg BW, respectively. The results from the acute toxicity test, bone marrow cell micronucleus test, and sperm morphology test were all negative, which implied that flavonoid-propionic acid mixtures from banana peel showed no mutagenic effects on somatic and germ cells and that they did not show indirect or direct mutagenicity. Therefore the mixtures are non-toxic and can be used as preservatives.

广东省广州市科技计划项目（2013J4100020）