苦丁茶叶制虫茶粗多酚的抗氧化、抗突变和体外抗肝癌效果

Antioxidant, Antimutagenic and in vitro Anticancer Effects of Crude Polyphenols in Insect Tea Made from Kuding Tea Leaves

摘要:

本实验对苦丁茶叶制虫茶粗多酚（KMICP）的抗氧化、抗突变效果和对人肝癌SMMC-7721细胞体外抗癌效果进行了观察。25、50、75和100 μg/mL浓度KMICP处理下，对DPPH自由基的清除能力分别为27.8%，46.6%，85.9%和99.8%。1.25和2.5 mg/皿浓度的KMICP对MNNG（N-甲基-N'-硝基-亚硝基胍）诱发突变表现出的49.5%和78.1%的抗突变能力，对AFB1（黄曲霉毒素B1）诱发突变的抑制效果也达到54.8%和76.4%。KMICP对体外生长的SMMC-7721细胞也表现出了明显的抑制作用，浓度达到150 μg/mL后，癌细胞的生长被完全抑制。通过RT-PCR检验可以观察到KMICP处理SMMC-7721细胞后，癌细胞的Bax、IκB-α、TIMP-1和TIMP-2表达上升，Bcl-2和NF-κB表达下降，且高浓度的KMICP较低浓度对基因表达的影响更为明显。这些实验结果充分的说明了KMICP具有很好的体外抗氧化、抗突变和抗癌效果。

Abstract:

In this study, the antioxidant, antimutagenic and in vitro anticancer effects of crude polyphenols in insect tea made from Kuding tea leaves (KMICP) on human hepatoma cells SMMC-7721 were investigated. The DPPH radical scavenging activity after treatment using 25, 50, 75, and 100 μg/mL KMICP was 27.8%, 46.6%, 85.9%, and 99.8%, respectively. KMICP at concentrations of 1.25 and 2.5 mg/plate showed antimutagenic effects on N-methyl-N'-nitro-N-nitrosoguanidine (MNNG)-induced mutation (49.5% and 78.1%, respectively) and aflatoxin B1 (AFB1)-induced mutation (54.8% and 76.4%, respectively). KMICP also showed significant inhibitory activity on SMMC-7721 cells in vitro, while the growth of cancer cells was completely inhibited at 150 μg/mL concentration. By reverse-transcription polymerase chain reaction (RT-PCR) conducted on KMICP-treated SMMC-7721 cells, it was found that Bax, IκB-α, TIMP-1, and TIMP-2 gene expression in cancer cells was increased, Bcl-2 and NF-κB gene expression was decreased, and the effect of high-concentration KMICP was more significant than that of low-concentration KMICP. These results demonstrate that KMICP shows good in vitro antioxidant, antimutagenic, and anticancer properties.