黄参茎叶多酚对四氯化碳所致小鼠急性肝损伤的保护作用

The Hepatoprotective Effect of Polyphenols from Sphallerocarpus gracilis Stem Leaves against CCl4-induced Acute Hepatic Damage in Mice

摘要:

研究黄参茎叶多酚对CCl4所致小鼠急性肝损伤的保护作用，探讨其对CCl4肝损伤起保护作用的有效剂量。以不同剂量的黄参茎叶多酚（150、300、600 mg/kg BW?d）给予小鼠灌胃实验进行预防，连续21 d后，腹腔注射1%的CCl4 (V/V)菜籽油0.3 mL建立CCl4诱导小鼠肝损伤模型。测定血清谷丙转氨酶(ALT)、谷草转氨酶(AST)、总胆固醇(TC)、总甘油三酯(TG)、总胆红素(TBIL)含量，以及肝组织中超氧化物岐化酶(SOD)、丙二醛(MDA)、过氧化氢酶(CAT)水平，并进一步观察肝脏组织病理学变化。结果表明：黄参茎叶多酚对CCl4所致小鼠肝损伤具有一定的保护作用，能显著抑制CCl4所致肝损伤小鼠肝脏指数、脾脏指数、血清ALT、AST、TBIL水平以及肝脏MDA含量的升高，能提高肝脏抗氧化酶SOD和CAT的活性，其中300 mg/kg Bw?d剂量水平效果最佳。肝组织病理学检查结果进一步证实了该结果。

Abstract:

The aim of this study was to investigate the protective effect of polyphenols from Sphallerocarpus gracilis stem leaves on carbon tetrachloride (CCl4)-induced hepatic injuries, and to identify its effective dosage. The mice in the treatment group were administrated with the polyphenols from Sphallerocarpus gracilis stem leaves at doses of 150, 300, and 600 mg/kg body weight (BW)/day for 21 consecutive days. The liver injury model was established by intraperitoneal injection of 3 mL 1% CCl4 rapeseed oil (V/V). The alanine transaminase (ALT), aspartate transaminase (AST), total cholesterol (TC), total triglyceride (TG), total bilirubin (TBIL) contents in serum, and superoxide dismutase (SOD), malondialdehyde (MDA), and catalase (CAT) levels in liver were measured. Meanwhile, a hepatic pathological examination was conducted. The results of this study showed that the polyphenols from Sphallerocarpus gracilis stem leaves could, to some extent, protect mice from CCl4-induced liver injury. In addition, these polyphenols significantly prevent the increase in liver index spleen index, serum ALT, AST, TBIL, and liver MDA levels, caused by CCl4 in mice. These polyphenols could also markedly increase the activities of liver SOD and CAT. Among the tested doses, 300 mg/kg BW/day showed the best effect. Histopathological examinations further confirmed these results.