利用层层自组装技术，成功将氨基苯磺酸、纳米银和辣根过氧化物酶标记的青霉素抗体固定在玻碳电极表面。实现了聚氨基苯磺酸/纳米银放大型电化学免疫传感器的制备，建立了一种高灵敏度检测青霉素的电化学分析方法。辣根过氧化物酶催化过氧化氢氧化还原3,4-二氨基苯甲酸产生电流信号，进行电化学检测，实现蜂蜜中青霉素的定量测定。同时对测定缓冲液的pH、反应温度和时间等进行了优化，最佳条件下，在0.05~6.00 μg/L浓度范围内传感器响应电流和青霉素浓度呈良好线性关系，线性方程为Δi(10-5A) = -0.7265C + 4.1522，相关系数为0.9946，检出限为1.02 μg/L。同时利用该传感器对蜂蜜样品中的青霉素进行了测定，测定结果与酶联免疫法进行了对比，可知该法测定青霉素灵敏度高，具有良好的选择性和稳定性，适用于检测蜂蜜样品中的青霉素残留。

Aminobenzene sulfonic acid, nano-Ag particles and horseradish peroxidase (HRP) labelled penicillin antibody was successfully immobilized on the glassy carbon electrode by the layer-by-layer assembly technique. The poly aminobenzene sulfonic acid/nano-Ag /HRP amplified electrochemical immunosensor was prepared and an enhanced sensitivity electroanalysis method for the detection of penicillin was established. HRP can catalyze the reduction of H2O2 to promote the oxidation of 3,4-diaminobenzonic acid, causing the changes of the peak current, thus realizing the detection of penicillin by the current changes. The effects of buffer solution pH，reaction temperature and time were investigated. Under optimized conditions, the current response of the sensor showed a good linear relationship with the concentration of penicillin within 0.05~6.00 μg/L, with a correlation coefficient of 0.9946 and the detection limit of 1.02 μg/L. The penicillin content in the honey samples was also detected by the modified electrode, and the detection results were compared with the traditional ELISA method. It is proved that this method has good sensitivity and specificity and suitable for detection of penicillin residues in honey samples.

滨州学院博士基金(2009Y02)；滨州市科技厅资助项目(2013ZC1603)