本文以卡拉胶/魔芋胶为材料，通过添加纳米TiO2/SiO2和超声波分散对其进行一定的修饰，采用电子扫描显微镜（SEM）和红外光谱分析（FTIR）进行了结构表征，研究了纳米复合涂膜对双孢菇在贮藏过程中生理品质指标的影响。结果表明：经超声波分散的卡拉胶/魔芋胶/纳米TiO2/SiO2复合膜其纳米粒子分散性较好，SiO2粒子与卡拉胶、魔芋胶表面的大量羟基存在强烈的氢键作用。贮藏12 d后，纳米复合涂膜处理组V的白度值，硬度值，可溶性固形物含量分别为87.75（菇肉）和82.48（菇皮）、9.00×105 Pa、6.90%，分别高于对照组的白度值84.22（菇肉）和78.48（菇皮）、硬度值7.60×105 Pa、可溶性固形物含量5.30%。经超声波处理的卡拉胶/魔芋胶/纳米TiO2/SiO2复合膜延缓了双孢菇失重率和相对电导率的升高，显著抑制了过氧化物酶（POD）的活性，推迟了多酚氧化酶（PPO）活性高峰的出现，对双孢菇的保鲜有明显效果。

In this study, carrageenan/konjac glucomannan films were modified by addition of nano TiO2 / SiO2 and ultrasonic dispersion. These films were characterized by Fourier transform infrared (FT-IR) spectroscopic analysis and scanning electron microscopy (SEM).Effects of the modified nano-composite film on postharvest physiology and quality of Agaricus bisporus during storage at 2 ℃ were studied. The results showed that nanoparticles in the carrageenan/konjac glucomannan/nano-TiO2 composite film, subjected to ultrasonic treatment were highly dispersed. There were a great number of hydrogen bonds between nano-sized SiO2 particles and hydroxyl groups on the surface ofcarrageenan/konjac glucomannan. After 12 d of storage, the values of whiteness, firmness of flesh, and soluble solid content (SSC) of Agaricus bisporus treated with the modified composite films were 87.75 (flesh) and 82.48 (skin),9.0×105 Pa and 6.9%, respectively, which were significantly higher than those of the control group (84.22 (flesh) and78.48 (skin), 7.6×105 Pa, and 5.3%). In addition, the modified carrageenan / konjac glucomannan /nano-SiO2 composite films delayed the increase in relative conductivity as well as weight lossin Agaricus bisporus, significantly inhibited the activity of peroxidase (POD) and delayed the peak of polyphenoloxidase (PPO) activity, thus remarkably improving the preservation of Agaricus bisporus.

国家自然科学基金资助项目(30871757；31301570)；科技部农业科技成果转化资金资助项（2010GB2C600253）