基于AEDA和OAV值确定西瓜汁香气活性化合物的比较

Identification of Aroma-active Compounds in Watermelon Juice by AEDA and OAV Calculation

摘要:

本研究采用溶剂辅助风味蒸发提取西瓜汁的香气成分，利用气相色谱-质谱联用结合嗅闻仪定性定量分析其香气活性化合物，共鉴定出49种化合物，主要是小分子的酮、醛、硫醚等物质，酯类物质中只检测到一种物质，即乙酸乙酯，且浓度较低。本研究首次检测到醚类物质。OAV计算和AEDA两种方法共同鉴定出11中物质，顺，顺-3, 6-壬二烯醛、顺-3-壬烯-1-醇、反，顺-3, 6-壬烯-1-醇、反-2-壬烯醛、壬醛、1-壬醇、2-戊基呋喃、己醛、辛醛、顺-6-壬烯醛、反-2-辛烯醛。其中有五种香气活性物质，即顺，顺-3, 6-壬二烯醛、顺-3-壬烯-1-醇、顺-6-壬烯醛、顺-2-壬烯醛、反, 顺-3, 6-壬烯-1-醇都能被实验员嗅闻到，且具有较高的OAV值。这表明，AEDA和OAV计算在西瓜汁香气活性化合物分析上具有一定的相似性。

Abstract:

Solvent assisted flavor evaporation and gas chromatography?mass spectrometry?olfactometry (GC-O-MS) were used in this study to identify aromatic compounds in watermelon juice. Forty-nine volatiles were identi?ed, most of which were low molecular weight alcohols, aldehydes and sulfur-containing compounds. There was only one ester that detected in melon juice, considered as ethyl acetate with low concentration. Sulfur-containing compounds were identified for the first time as aroma compounds in watermelon. Eleven compounds, namely (Z)-3-Nonen-1-ol, (Z, Z)-3,6-nonadienal, (E,Z)-3,6-noandienol, (E)-2-nonenal, nonanal, 1-nonanol, 2-pentylfuran, hexanal, Octanal, (Z)-6-nonenal and (E)-2-octenal, were identi?ed as the potent odorants in watermelon juice by both odor active value(OAV) and aroma extract dilution analysis(AEDA). In addition, five odorants including (Z)-3-Nonen-1-ol, (Z, Z)-3, 6-nonadienal, (E, Z)-3, 6-noandienol, (Z)-6-nonenal, (Z)-2-nonenal were detected by all of the panelists and showed higher OAVs, indicating that AEDA and OAV resulted in relatively similar watermelon aroma patterns.