通过单因素分析，对不同溶剂、温度和时间条件下提取葡萄藻微量藻粉中碳氢化合物的效果进行了比较，获得了最佳提取条件为：40 mg藻粉悬浮于正己烷中，超声处理（35 ℃，70 Hz）半小时后再于35 ℃下提取10 h，碳氢化合物得率最高，占藻粉干重的2.95‰。比较获得了GC-MS分析的程序升温优化条件，发现处于对数期的葡萄藻微量冻干藻粉中含量最高的碳氢化合物都是C22-C31长链烷烃，其组成与含量分别为：二十二烷（50.40 ?g/g）、二十四烷（209.17 ?g/g）、二十五烷（351.45 ?g/g）、9-辛基十七烷（472.38 ?g/g）、二十七烷（540.48 ?g/g）、二十八烷（542.24 ?g/g）、角鲨烯（473.60 ?g/g）、正三十一烷（306.86 ?g/g）。该提取和分析过程具有样品量少、时间短、得率高和操作简单的优点，可作为葡萄藻碳氢化合物分析检测的一种快速高效途径。

Based on ANOVA analysis of the solvents, temperatures and time, the optimal conditions for hydrocarbons extraction from small amount of algal powder of Botryococcus sudeticus were obtained as follows: 40 mg algal powder suspended in n-hexane was ultrasonicated at 35 ℃ and 70 Hz for 30 min, then extracted at 35 ℃ for 10 hours. The highest yield of hydrocarbons accounted for 2.95 ‰ of dry weight was obtained under above conditions. The programmed procedure for GC-MS analysis was compared and optimized, and long-chain alkanes from C22 to C31 were found as the dominant hydrocarbons in the lyophilized powder of Botryococcus sudeticus from the cells in exponential phase. They were consisted of docosane, tetracosane, pentacosane, 9-octylheptadecane, heptacosane, octacosane, squalene and hentriacontane, with contents of 50.40, 209.17, 351.45, 472.38, 540.48, 542.24, 473.60 and 306.86 μg/g of dry weight, respectively. This extraction and analysis process have advantages of fewer amounts of sample required, time-saving, high yield and simple operation, which is a rapid and high efficient method for the analysis and detection of hydrocarbons from Botryococcus sudeticus.

国家973项目（2011CB200901）；国家863计划项目（2013AA065802）；国家自然科学基金（31370383）；国家海洋局海洋可再生能源专项资金资助项目（GHME2011SW04）