类芽孢杆菌YS-1粗提液对南丰蜜橘贮藏效果的影响

Effects of Paenibacillus brasilensis YS-1 Crude Extracts on Storage of Nanfeng Mandarin

摘要:

本文以南丰蜜橘“小果97-2”为研究对象，利用类芽孢杆菌Paenibacillus brasilensis YS-1粗提液处理果实，科力鲜500倍水溶液和清水处理为对照，研究它们在冷藏（5±1 ℃）条件下对南丰蜜橘果实采后生理生化及贮藏效果的影响。结果表明，YS-1粗提液与科力鲜对意大利青霉的离体抑菌圈直径分别为20.20 cm和51.12 cm；冷藏100 d后，YS-1粗提液处理的果实腐烂率和失重率分别为20.67%和3.75%，显著低于清水对照，高于科力鲜；同时，YS-1粗提液处理使南丰蜜橘果实TSS、总糖和Vc含量峰值提高至15.70%、19.57%和24.58%，减缓了贮藏后期南丰蜜橘果实TSS、总糖和TA含量的下降，有效保持了果实贮藏品质。此外，YS-1粗提液处理在贮藏中后期可以抑制果实丙二醛的积累和呼吸作用的增强，提高果实SOD、POD以及PAL活性。表明YS-1粗提液处理能有效保持南丰蜜橘果实贮藏品质，提高果实的抗病能力，减缓果实的衰老。

Abstract:

The fruits of Nanfeng mandarin “97-2” were treated with the crude extracts of Paenibacillus brasilensis YS-1, then stored at 5±1 ℃ for 100 days. The postharvest physiology and storability of fruits were investigated and compared with 500-fold dilution of an antistaling agent named “Kelixian” treatment and distilled water as control. The result showed that the treatment of crude extracts of YS-1 and 500-fold dilution of “Kelixian” effectively inhibited the growth of P. intalicum, and the inhibition diameters were 20.20 cm and 51.12 cm respectively. The rotting rate and weight loss rate of fruits which treated with crude extracts of YS-1 were 20.67% and 3.75%, which significantly higher than distilled water but lower than “Kelixian” treatment. And the contents of total soluble solids, total sugars and Vc were increased to 15.70%、19.57% and 24.58%, respectively. It also delayed the decline rate of total soluble solids and contents of total sugars and titratable acids, while effectively kept the storage quality of the fruit. In addition, crude extracts of YS-1 treatment decreased respiration rate and MDA content in the middle and later periods of storage, and increased the SOD, POD and PAL activities. It indicated that crude extracts of P. brasilensis YS-1 treatment can keep the quality of fruit, improve the ability of disease resistance, and delay the fruit senescence of Nanfeng mandarin during storage.