阳澄湖所产的中华绒螯蟹风味优良，为我国传统名贵水产品，深受消费者喜爱。采用新型材料-固相萃取整体捕集剂（MonoTrap）作为吸附剂，在优化吸附剂种类、吸附剂个数、萃取温度和萃取时间的基础上，对阳澄湖中华绒螯蟹性腺中的挥发性成分进行了捕集，并结合气相色谱-质谱联用仪(GC-MS)对捕集的挥发性成分进行分离鉴定。结果表明，采用7个MonoTrap RCC18、萃取温度为100 ℃、萃取时间为50 min时萃取效果最佳，以此条件可从阳澄湖中华绒螯蟹性腺中鉴定得到七大类55种挥发性成分。查阅各挥发物的阈值并计算其气味活性值后，从55种挥发物中筛选得到了22种重要的“活性气味物质”，其中2,4-癸二烯醛、三甲胺、癸醛这3种物质对性腺样品的整体风味贡献最为显著。固相萃取整体捕集剂法对挥发性化合物吸附效率高，是一种优良的食品风味分析方法。

Eriocheir sinensis farmed in Yangcheng Lake is a valuable traditional aquatic product and quite welcomed by Chinese consumers due to its outstanding flavor. In this study, a new type of material (MonoTrap) was applied as the adsorbent to extract the volatile compounds in gonad of Eriocheir sinensis. The volatile compounds were then isolated and identified by GC-MS. The optimal extraction conditions were using seven MonoTrap RCC18 at 100 ℃ for 50 minutes. 55 volatiles that could be divided into seven classes were finally detected under the forementioned conditions. Odor activity values (OAV) of all 55 volatiles were calculated according to their odor thresholds and 22 aroma-active compounds (AACs) were further selected. Among the AACs, 2,4-decadienal, trimethylamine and decanal mainly contributed to integral aroma profile of the gonad sample. Monolithic Material Sorptive Extraction (MMSE) is considered as an excellent method for food flavor analysis because of its high efficiency of volatile compounds absorption.

上海市中华绒螯蟹产业技术体系建设项目(D-8003-10-0208)；上海市教委“食品质量与安全”重点学科建设项目(J50704)；上海海洋大学优秀研究生论文培育计划项目(B-9600-10-0003-3)