芹菜素的制备、鉴定与纯化的研究

Preparation, Identification and Purification of Apigenin

摘要:

芹菜素具有抗肿瘤、降血压等多种生理活性功能，在功能性食品和医药领域极具应用前景，但由于传统的芹菜素生产是以洋苷菊和旱芹为原料，其芹菜素的含量很低，这就导致芹菜素的生产得率低，生产成本高，限制了其广泛应用。亮叶杨桐是我国特有的植物，其叶富含以芹菜素为苷元的类黄酮物质，本文研究了以亮叶杨桐叶为原料制备芹菜素的方法。通过向亮叶杨桐叶的水提液中加入一定量的硫酸，加热酸解，可获得大量沉淀物，其主要的成分经紫外、红外、电喷雾质谱及核磁分析，鉴定为芹菜素，液相分析表明该粗品中芹菜素含量为64.80%。通过测定该粗品中芹菜素在不同温度，不同浓度的乙醇中溶解能力的分析，本研究确定采用恒温浓度梯度法精制芹菜素，获得的芹菜素纯度为92.44%。

Abstract:

Apigenin possesses many bioactivities such as antitumor and antihypertension, which can be widely used in functional food and medicine. Apigenin was used to be prepared from Matricaria recutita and Apium graveolens. Their low contents of apigenin led the low yield and high cost of apigenin, which limited the extensive application of apigenin. Adinandra nitida is a special plant in China, whose leaves is rich in flavonoids with apigenin as aglycone. The preparation of apigenin from Adinandra nitida leaves was studied. The water extract of Adinandra nitida leaves was heated and hydrolyzed with sulfuric acid. The main component of the obtained precipitate was identified as apigenin by UV, IR, ESI-MS and NMR. And the content of apigenin in the precipitate was determined as 64.80 % by HPLC. The solubility of apigenin in the ethanol with different temperatures and concentrations was measured, and the constant temperature-concentration gradient technology was developed to purify apigenin. The purity of apigenin reached 92.44 %。