[关键词]
[摘要]
本研究发现,玉米芯能够使菌株CL-6生成葡萄糖淀粉酶的时间缩短为原来的1/3。于是,对菌株CL-6进行鉴定,并分析了玉米芯中的糖类成分以探究其中诱导因子的化学成分。菌株的鉴定采用形态学和ITS序列分子鉴定方法,葡萄糖淀粉酶酶活测定方法采用葡萄糖氧化酶法,发酵体系采用液体摇瓶发酵法,玉米芯中糖类成分的测定采用薄层层析和高效液相示差折光检测法。结果表明,菌株CL-6为米根霉属。玉米芯中水溶性成分中的糖类成分为蔗糖、葡萄糖、果糖和麦芽糖,其含量为520.33、242.67、228.67、30.33 mg/2.5 g湿基。通过分析在米根霉CL-6液体发酵体系中加入玉米芯、玉米芯水溶性成分、玉米芯水溶性成分中的糖类成分以及玉米芯水溶性成分中分子量高于8~14.4 KD成分对其葡萄糖淀粉酶酶活的影响,本研究推测,玉米芯中的诱导因子为分子量高于8~14.4 KD的水溶性物质而非糖类成分。
[Key word]
[Abstract]
The glucoamylase produced by strain CL-6 was two-fold hastened when the organism was grown on media containing corncob. Strain CL-6 isolated from sweet starter was identified by morphological observation and ITS sequence analysis and indicated as Rhizopus oryzae. The enzymatic activity was measured by glucose oxidase-peroxide enzyme (GOD-POD). The sugar components in corncob were determined by thin-layer chromatography (TLC) and HPLC-RID. The sugars in the water-soluble components of the corncob were as follows: sucrose(520.33 mg/2.5 g wet resin), glucose (242.67 mg/2.5 g wet resin), fructose(228.67 mg/2.5 g wet resin) and maltose(30.33 mg/2.5 g wet resin). The effect of separately adding corncob, soluble fractions of corncob, sugar components and soluble fractions with molecular weight higher than 8~14.4 KD on the activity of glucoamylase were analyzed It was showed that glucoamylase formation was not enhanced when the organisms grew on media containing the four sugars, and the components with molecular weight exceeding 8~14.4 KD in the water-soluble parts of corncob was the important factor in stimulating glucoamylase prodution by Rhizopus oryzae CL-6.
[中图分类号]
[基金项目]