本文筛选了能够抑制食品中典型腐败酵母的乳酸菌，研究其抑制特性并进行了分子生物学鉴定。从泡菜，榨菜、酸奶、火腿等食品中分离得到乳酸菌57株，通过双层拮抗平板法筛选能够抑制食品中典型腐败酵母（假丝酵母属、毕赤酵母属，酵母属、蔷薇酵母属和解脂耶罗维亚酵母）的乳酸菌6株，其中菌株AT 6能够抑制所有11株腐败酵母指示菌。96孔板法测定乳酸菌AT6发酵上清液对腐败酵母的抑制率可达73.04%以上，其抑制活性对蛋白酶不敏感，但随着pH值的升高而降低。菌株AT6在MRS培养基中的生长曲线研究表明30 ℃静置培养30 h，其发酵上清液可达到对白色念珠菌的最大抑菌活性95.02%。提取菌株AT6基因组并扩增16S rRNA基因，将PCR片段克隆到pMD 19-T 载体后进行测序，BLAST分析和多重比对后确定AT6为食窦魏斯氏菌。

Lactic acid bacteria (LAB) with inhibition activity against food-borne spoilage yeast were screened, characterized and identified by molecular biological method. Fifty-seven LAB strains were isolated from different food products such as pickles, pickled mustards, yoghourt and hams. Overlay method showed that six LAB strains represented inhibition activity against food-borne spoilage yeast such as Candida, Pichia, Saccharomyces, Rhodotorula and Yarrowia lipolytica. Among them, strain AT6 could inhibit the growth of all eleven spoilage yeast indicators. 96 well-plate method showed that the inhibition rate of supernatant AT6 reached above 73.04%. The supernatant AT6 was not sensitive to proteinase but lose the activity at higher pH value. The inhibition activity of AT6 supernatant against Canidia albicans was maximal after 30 h incubation at 30 ℃. The genome of AT6 was extracted and the gene of 16S rRNA was amplified by PCR. The PCR products were cloned into pMD 19-T T-vector and sequenced. The sequence was BLISA and strain AT6 was identified as Weissella cibaria.

西华大学重点科研基金项目（Z1120536）