研究了不同溶剂普洱茶提取物及萃取物的体外抑菌活性。论文采用滤纸片扩散法和最低抑菌浓度检测了水提、75%和95%乙醇普洱茶提取物对S. aureus、E. coli、P. aeruginosa及B. subtilis体外抑菌效果。同时分析了95%醇提物的不同溶剂萃取物对供试菌的影响。结果表明：95%乙醇浸提物抑菌效果最好，尤其对S. aureus、E. coli最为明显，抑菌圈分别为19.58±0.24 mm、14.58±0.24 mm，MIC分别为0.63 mg/mL，0.39 mg/mL，但对B. subtilis抑菌效果差。在95%醇提物的不同溶剂萃取物中，萃取物抑菌活性为：乙酸乙酯>正丁醇>石油醚>氯仿。乙酸乙酯萃取物对S. aureus抑制效果最好，其抑菌圈直径为31.88±0.24 mm，MIC为0.47 mg/mL。普洱茶提取物的醇提乙酸乙酯萃取提物能显著抑制革兰氏阴性菌和阳性菌的生长，研究结果为普洱茶抗菌活性成分的开发利用提供一定的实践和数据参考。

The antibacterial activity of the solvent extracts from Pu’er tea in vitro was studied in this paper. Filter paper disc diffusion method and minimal inhibition concentrations (MIC) were employed to determine the antibacterial activities of Pu’er tea extracts by water, 75% and 95% ethanol on S. aureus, E. coli, P. aeruginosa and B. subtili s. The 95% ethanol extracts showed the highest antibacterial effect, especially to S. aureus and E. coli.with the inhibition radius being of 19.58 ± 0.24 mm and 14.58 ± 0.24 mm, respectively. The MIC was 0.63 mg/mL, 0.39 mg/mL. But 95% ethanol extracts showed little inhibitory effect on the growth of B. subtilis. The extracts obtained using different solvent showed different antibacterial effects, amont which the ethyl acetate extracts showed the strongest antibacterial activities, followed with the extracts of n-butyl alcohol, petroleum ether and chloroform. For different bacteria tested, the ethyl acetate extracts showed the strongest effects on the growth of S. aureus with the inhibition zone diameter being of 31.88 ± 0.24 mm and the MIC being of 0.473 mg/mL. The ethyl acetate extract of Pu'er tea could significantly inhibit the growth of gram-positive and gram-negative bacteria. This study provided the references for the development and utilization of antibacterial active ingredients from Pu'er tea.

国家自然科学基金资助项目（31060270）；云南省自然科学基金（2011FZ091）