本文研究了AB-8、D101、HP-20三种大孔吸附树脂对荷叶总生物碱的动态、静态吸附及解吸性能，筛选出一种高吸附、高解吸性能树脂。在此基础上研究了吸附流速、上样液pH值、最大上样量、解吸流速、洗脱液浓度及pH值对提取、纯化荷叶碱的影响。结果表明：D101树脂为提取、纯化荷叶总生物碱的最佳树脂，其最佳纯化条件是：吸附流速2 BV/h，上样液pH值10，最大上样量6 BV；解吸流速2 BV/h，洗脱液为pH值3的70%乙醇溶液。荷叶总生物碱纯化物中荷叶碱的含量为2.26%。为荷叶总生物碱的开发利用打下了基础。

For purification total alkaloids of Nelumbonucifera leaves, capability of adsorption and desorption of three kinds of macroporous adsorption resin such as AB-8, D101 and HP-20 were explored. Then the effects of adsorption parameters, such as flow rate of adsorption, pH value, maximum loading amount of the extract solution; concentration, pH value of eluant and flow rate of desorption, on the nuciferine content were investigated by HPLC. The results showed that D101 was the most efficient Resin. The optimum condition were 2BV/h of loading rate, 10 of pH value, 6 BV of maximum loading amount of the extract solution, flow rate of desorption 2BV/h and pH 3 of the eluent (70% aqueous ethanol solution). Under such conditions, the ratio of nuciferine to the purified extract of Nelumbonucifera leaves was up to 2.26%. This research provided references for comprehensive development and utilization of the total Alkaloids of Leaves of Nelumbonucifera Gaertn.