三种菊科植物抗癌抗炎组分分离与功能研究

Anti-cancer and Anti-inflammatory Functions of Active Components Isolated from Three Compositaes

摘要:

本研究以甜叶菊、蒲公英、野菊花三种菊科植物为材料，采用水、乙醇/乙酸乙酯、甲醇和正己烷4种溶剂提取，硅胶柱层析、TLC分离纯化，以小鼠肝癌细胞Hepa 1c1c7和小鼠巨噬细胞Raw 264.7为模型，通过测定醌还原酶活性和一氧化氮抑制率，筛选具有抗癌和抗炎的活性组分。结果：甜叶菊、蒲公英、野菊花的乙醇/乙酸乙酯提取物经分离纯化后得到最强活性组分，其诱导醌还原酶倍增的浓度分别为0.26~1.59 μg/mL、0.45~3.73 μg/mL和0.60~0.92 μg/mL；甜叶菊、蒲公英组分一氧化氮抑制率达到50%的浓度分别为14.02~19.04 μg/mL、48.90~86.05 μg/mL。这一研究结果为深入开展菊科植物抗癌抗炎功能成分的分离鉴定及其作用机理打下了前期研究基础。

Abstract:

The anti-cancer and anti-inflammatory functions of active components from Stevia Rebaudiana, Taraxacum Officinale and Chrysanthemum Indicum L. were investigated in this article. The components extracted with four solvents (water, ethanol/ethyl acetate, methanol and n-hexane) were separated and purified by silica gel column chromatography and TLC. Murine hepatoma cells (Hepa 1c1c7) and mouse macrophage cells (Raw 264.7) were used to determine quinone reductase activity and inhibition of nitric oxide. Results showed that the strongest activity for all three species was achieved by the ethanol/ethyl acetate extracting; the minimal concentrations to increases double QR specific activity were 0.26~1.59 μg/mL, 0.45~3.73 μg/mL and 0.60~0.92 μg/mL, respectively, for Stevia Rebaudiana, Taraxacum Officinale and Chrysanthemum Indicum L.. The minimal concentrations of Stevia Rebaudiana and Taraxacum Officinale to achieve 50% inhibition of nitric oxide were 14.02~19.04 μg/mL and 48.90~6.05 μg/mL, respectively. The results provided preliminary basis for identification of anti-cancer and anti-inflammatory ingredients and mechanical investigations from the compositae.