建立同时测定小根蒜中尿苷、鸟苷和腺苷含量的反相高效液相色谱（RP-HPLC）梯度洗脱法。采用ZORBAX-SB-C18色谱柱(150 mm×4.6 mm, 5 μm)；以水-甲醇为流动相，梯度洗脱，流速为0.6 mL/min，柱温为25 ℃；检测波长：260 nm。尿苷在0.0784~0.784 μg范围内线性关系良好, 线性相关系数r=0.9995；鸟苷在0.0798~0.798 μg范围内线性关系良好，线性相关系数r=0.9995；腺苷在0.0852~0.852 μg范围内线性关系良好，线性相关系数r=0.9994。该法操作简便，方法可靠，重现性好，可以作为小根蒜的质量控制方法之一。

A new method for determination of uridine, guanosine and adenosine contents in Allium macrostemon bunge were established by using RP-HPLC gradient elution. The extracts were separated on a ZORBAX-SB-C18 analytical column (4.60 mm×250 mm, 5 μm) with CH3OH-H2O as mobile phase at 260 nm. The flow rate was 0.6 mL?min- 1and the column temperature was 25 ℃. The linear ranges of uridine,guanosine and adenosine were 0.0784~0.784 μg（r=0.9995）, 0.0798~0.798 μg（r=0.9995）and 0.0852~0.852 μg (r=0.9994), respectively. This method showed high reproducibility and can be used for quality control of the Allium macrostemon bunge.

贵州省科技创新人才团队项目(合同号：黔科合人才团队（2010）4011号)