采用反相高效液相色谱测定烟草中的茄尼醇，采用外标法进行定量，确定了色谱分离的条件，并通过皂化提取实验确定了茄尼醇最佳皂化条件：58 ℃、0.6 mol/L KOH甲醇溶液皂化35 min。该法具有良好的线性（相关系数为0.9999），检测限为27.4 ng（S/N=3），加标回收率为99 %，方法的RSD为0.41%，方法简单、准确。

A simple and rapid method was developed for determination of solanesol in tobacco by RP-HPLC and the saponification process was optimized. The solanesol content was quantitatively determined with the external standard method. The results indicated that the optimum saponification process was as follows: KOH -methanol solution 0.5 mol/L, of saponification temperature 30 ℃, and the saponification time 30 minutes. The method had good linearity with correlation coefficient of 0.9999. The limit of detection based on signal-to-noise ratio of 3 was 27.4 ng. The recovery of solanesol in tobacco was 99% with RSD of 0.41%.