为了鉴定大豆异黄酮的结构，采用高效液相色谱-电喷雾离子源-离子阱多级质谱联用仪(HPLC-ESI-MSn)法分离鉴定大豆粉乙醇提取物，通过多级质谱提供的准分子离子峰和多级碎片离子信息，分析得到了12种大豆异黄酮的相对分子质量、保留时间并推断了它们异黄酮糖苷的组成结构、异黄酮糖苷中糖的类型等，这12种大豆异黄酮分别是大豆苷元、大豆黄素、染料木素、大豆苷、大豆黄苷、染料木苷、乙酰基大豆苷、乙酰基大豆黄苷、乙酰基染料木苷、丙二酰基大豆苷、丙二酰基大豆黄苷、丙二酰基染料木苷。

To determine the construction of soybean isoflavone, high performance liquid chromatography-electropray ionization multi-stage tandem mass spectrometry（HPLC-ESI-MSn） was successfully applied to analysis and determine ethanol extracts of soybean meal. Thouth this method, the accurate molecular ion peak and multistage fragment ion information were provided. Relative molecular mass, retention time and constructions of flavoneid aglycones of 12 kinds of soybean isoflavones were analyzed. The types of glycogen in isoflavone were also discussed. The 12 kinds of soybean isoflavone glycosides were identified as daidzein, glycitein, genistein, daidzin, glycitin, genistin, 6"-o-acetyldaidzin, 6"-o-acetylglycitin, 6"-o-acetylgenistin, 6"-o-malonyldaidzin, 6"-o-malonylglycitin and 6"-o-malonylgenistin.

农业部转基因专项（2011ZX08012-001），“863”计划课题（2012AA101604），天津市食品营养与安全重点实验室建设（12TXSYJC33600）